Active Recombinant Human PARG protein, His-tagged

• Cat.No.: PARG-31H
• Product Overview: Recombinant Human PARG fussed with His tag was expressed in Sf21 cells.

Specification

• Description: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. PARG hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
• Bio-activity: ~100ng is required for poly(ADP-ribose) degradation assays.
• Purity: ≥95% (SDS-PAGE)
• Notes: After opening, prepare aliquots and store at -80 centigrade. Avoid freeze/thaw cycles.
• Stability: Stable for at least 6 months after receipt when stored at -80 centigrade.
• Storage: Short Term Storage: -20 centigrade;Long Term Storage: -80 centigrade.
• Concentration: Lot dependent (0.2-1.5mg/ml)
• Publication:
  A Study into the ADP-Ribosylome of IFN-γ-Stimulated THP-1 Human Macrophage-like Cells Identifies ARTD8/PARP14 and ARTD9/PARP9 ADP-Ribosylation (2019)
  A Novel Spectral Annotation Strategy Streamlines Reporting of mono-ADP-ribosylated Peptides Derived from Mouse Liver and Spleen in Response to IFN-γ (2021)

Gene Information

• Gene Name: PARG poly (ADP-ribose) glycohydrolase [ Homo sapiens ]
• Official Symbol: PARG
• Synonyms: PARG99; poly(ADP-ribose) glycohydrolase; mitochondrial poly(ADP-ribose) glycohydrolase; poly(ADP-ribose) glycohydrolase 60 kDa isoform
• Gene ID: 850
• mRNA Refseq: NM_003631
• Protein Refseq: NP_003622
• MIM: 603501
• UniProt ID: Q86W56
• Chromosome Location: 10q11.23
• Pathway: Base Excision Repair, organism-specific biosystem; DNA Repair, organism-specific biosystem; POLB-Dependent Long Patch Base Excision Repair, organism-specific biosystem
• Function: poly(ADP-ribose) glycohydrolase activity