Active Recombinant Human CD33, Fc-tagged, Biotinylated
Cat.No. : | CD33-675H |
Product Overview : | The recombinant human CD33-Fc is expressed as a 471 amino acid protein consisting of Asp18 - Gly260 region of CD33 and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition. |
- Specification
- Gene Information
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Source : | Human cells |
Species : | Human |
Tag : | Fc |
Form : | Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule). |
Bio-activity : | Immobilized protein supports the adhesion of human red blood cells. Binds anti-CD33/SIGLEC-3 monoclonal antibody, human IgG1 with high affinity (KD< 10 nM) by a functional ELISA. |
Molecular Mass : | Calculated molecular mass (kDa): 52.5; Estimated by SDS-PAGE under reducing condition (kDa): 65-75 (probably due to glycosylation) |
AA Sequence : | DPNFWLQVQESVTVQEGLCVLVPCT FFHPIPYYDKNSPVHGYWFREGAII SRDSPVATNKLDQEVQEETQGRFR LLGDPSRNNCSLSIVDARRRDNGSY FFRMERGSTKYSYKSPQLSVHVTDL THRPKILIPGTLEPGHSKNLTCSV SWACEQGTPPIFSWLSAAPTSLGPR TTHSSVLIITPRPQDHGTNLTCQVK FAGAGVTTERTIQLNVTYVPQNP TTGIFPGDGSGKQETRAGVVHGSTG THTCPPCPAPELLGGPSVFLFPPKP KDTLMISRTPEVTCVVVDVSHEDP EVKFNWYVDGVEVHNAKTKPREEQY NSTYRVVSVLTVLHQDWLNGKEYKC KVSNKALPAPIEKTISKAKGQPR EPQVYTLPPSREEMTKNQVSLTCLV KGFYPSDIAVEWESNGQPENNYKTT PPVLDSDGSFFLYSKLTVDKSRWQ QGNVFSCSVMHEALHNHYTQKSLSL SPGK |
Endotoxin : | <0.1 eu per 1 μg of purified recombinant protein determined by the lal>0.1> |
Purity : | >95% judged by SDS-PAGE under reducing condition |
Storage : | The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles. |
Gene Name : | CD33 CD33 molecule [ Homo sapiens ] |
Official Symbol : | CD33 |
Synonyms : | CD33; CD33 molecule; CD33 antigen (gp67); myeloid cell surface antigen CD33; FLJ00391; p67; sialic acid binding Ig like lectin 3; SIGLEC 3; SIGLEC3; gp67; sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; SIGLEC-3; |
Gene ID : | 945 |
mRNA Refseq : | NM_001082618 |
Protein Refseq : | NP_001076087 |
MIM : | 159590 |
UniProt ID : | P20138 |
Chromosome Location : | 19q13.3 |
Pathway : | Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; |
Function : | receptor activity; sugar binding; |
Products Types
◆ Lysates | ||
CD33-978HCL | Recombinant Human CD33 cell lysate | +Inquiry |
CD33-1808MCL | Recombinant Mouse CD33 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionCertain polymorphisms in the CD33 gene can influence its expression on the cell surface, which might affect the efficacy of CD33-targeted therapies.
CD33 is a transmembrane receptor primarily expressed on cells of myeloid lineage and is involved in modulating cellular functions, including the regulation of cellular proliferation and differentiation.
CD33 is predominantly expressed on myeloid cells, especially on immature myeloid cells. Its expression decreases as these cells differentiate and is typically low or absent on lymphoid cells.
CD33 has been targeted for therapeutic antibodies in AML. An example is gemtuzumab ozogamicin, which targets CD33 and delivers a cytotoxic agent specifically to leukemia cells expressing CD33.
While the exact signaling pathways of CD33 are still under investigation, it is believed that CD33 signaling can influence the differentiation and function of myeloid cells, potentially playing a role in immune regulation.
CD33 is commonly expressed on the surface of AML blasts, making it a potential target for therapeutic interventions in AML.
The specific ligands for CD33 are not fully characterized, but it is believed to play a role in cell-cell interactions within the immune system.
Customer Reviews (3)
Write a reviewQuick dissolution, easy use.
Excellent solubility, no aggregates.
High concentration, very effective.
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