Active Recombinant Human TIE1, Fc-tagged, Biotinylated

• Cat.No.: TIE1-700H
• Product Overview: The recombinant human Tie1-Fc fusion is expressed as a 967 amino acid protein consisting of Ala22 - Gln759 region of Tie1 and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Specification

• Source: Human cells
• Species: Human
• Tag: Fc
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Interacts with Tie2 and inhibits angiopoietin/Tie2-mediated signaling activity.
• Molecular Mass: Calculated molecular mass (kDa): 105.5; Estimated by SDS-PAGE under reducing condition (kDa): 110-120
• AA Sequence: LSARVHKEKQTDVIWKSNGSYFYTL DWHEAQDGRFLLQLPNVQPPSSGIY SATYLEASPLGSAFFRLIVRGCGA GRWGPGCTKECPGCLHGGVCHDHDG ECVCPPGFTGTRCEQACREGRFGQS CQEQCPGISGCRGLTFCLPDPYGC SCGSGWRGSQCQEACAPGHFGADCR LQCQCQNGGTCDRFSGCVCPSGWHG VHCEKSDRIPQILNMASELEFNLE TMPRINCAAAGNPFPVRGSIELRKP DGTVLLSTKAIVEPEKTTAEFEVPR LVLADSGFWECRVSTSGGQDSRRF KVNVKVPPVPLAAPRLLTKQSRQLV VSPLVSFSGDGPISTVRLHYRPQDS TMDWSTIVVDPSENVTLMNLRPK TGYSVRVQLSRPGEGGEGAWGPPTL MTTDCPEPLLQPWLEGWHVEGTDRL RVSWSLPLVPGPLVGDGFLLRLWD GTRGQERRENVSSPQARTALLTGLT PGTHYQLDVQLYHCTLLGPASPPAH VLLPPSGPPAPRHLHAQALSDSEI QLTWKHPEALPGPISKYVVEVQVAG GAGDPLWIDVDRPEETSTIIRGLNA STRYLFRMRASIQGLGDWSNTVEE STLGNGLQAEGPVQESRAAEEGLDQ GSTGTHTCPPCPAPELLGGPSVFLF PPKPKDTLMISRTPEVTCVVVDVS HEDPEVKFNWYVDGVEVHNAKTKPR EEQYNSTYRVVSVLTVLHQDWLNGK EYKCKVSNKALPAPIEKTISKAK GQPREPQVYTLPPSREEMTKNQVSL TCLVKGFYPSDIAVEWESNGQPENN YKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCSVMHEALHNHYTQK SLSLSPGK
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the lal>
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: TIE1 tyrosine kinase with immunoglobulin-like and EGF-like domains 1 [ Homo sapiens ]
• Official Symbol: TIE1
• Synonyms: TIE1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; TIE, tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; tyrosine-protein kinase receptor Tie-1; JTK14; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; TIE
• Gene ID: 7075
• mRNA Refseq: NM_001253357
• Protein Refseq: NP_001240286
• MIM: 600222
• UniProt ID: P35590
• Chromosome Location: 1p34-p33
• Function: ATP binding; nucleotide binding; protein binding; protein tyrosine kinase activity; receptor activity; transmembrane receptor protein tyrosine kinase activity