Recombinant Human AKR1A1 protein, His-tagged AKR1A1-129H

Recombinant Human AKR1A1 protein, His-tagged

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Recombinant Human AKR1A1 protein, His-tagged

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Cat.No. : AKR1A1-129H
Product Overview : Recombinant Human AKR1A1(1-325aa) fused with His tag was expressed in E. coli.
Description : Alcohol dehydrogenase [NADP+] (AKR1A1) is a member of the aldo/keto reductase superfamily and catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. This protein is closely related (65% identity) to aldose reductase, an enzyme involved in the pathogenesis of some diabetic and galactosemic complications. It plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Recombinant human Alcohol dehydrogenase was expressed in E. coli and purified by using conventional chromatography techniques.
Source : E. coli
Species : Human
Tag : His
Form : Supplied as a liquid in 20mM Tris-HCl (pH8.0)50mM NaCl, 10% glycerol.
Molecular Mass : 36.5 kDa
Protein length : 1-325aa
AA Sequence : MAASCVLLHT GQKMPLIGLG TWKSEPGQVK AAVKYALSVG YRHIDCAAIY GNEPEIGEAL KEDVGPGKAV PREELFVTSK LWNTKHHPED VEPALRKTLA DLQLEYLDLY LMHWPYAFER GDNPFPKNAD GTICYDSTHY KETWKALEAL VAKGLVQALG LSNFNSRQID DILSVASVRPAVLQVE CHPY LAQNELIAHC QARGLEVTAY SPLGSSDRAW RDPDEPVLLE EPVVLALAEK YGRSPAQILL RWQVQRKVIC IPKSITPSRI LQNIKVFDFT FSPEEMKQLN ALNKNWRYIV PMLTVDGKRV PRDAGHPLYP FNDPY
Purity : Affinity Purified~90% (SDS-PAGE) Purified by immunoaffinity chromatography.
Notes : Small volumes of alcohol dehydrogenase recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
Storage : Aliquot to avoid repeated freezing and thawing and freeze at -70 centigrade. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Gene Name : AKR1A1 aldo-keto reductase family 1, member A1 (aldehyde reductase) [ Homo sapiens ]
Official Symbol : AKR1A1
Synonyms : AKR1A1; aldo-keto reductase family 1, member A1 (aldehyde reductase); alcohol dehydrogenase [NADP(+)]; ALR; DD3; dihydrodiol dehydrogenase 3; aldehyde reductase; alcohol dehydrogenase; aldo-keto reductase family 1 member A1; ARM; ALDR1; MGC1380; MGC12529;
Gene ID : 10327
mRNA Refseq : NM_001202414
Protein Refseq : NP_001189343
MIM : 103830
UniProt ID : P14550
Chromosome Location : 1p33-p32
Pathway : Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; tryptophan degradation X (mammalian, via tryptamine), organism-specific biosystem;
Function : L-glucuronate reductase activity; alditol:NADP+ 1-oxidoreductase activity; electron carrier activity; oxidoreductase activity;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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