Recombinant Human AKR1A1 protein, His-tagged

• Cat.No.: AKR1A1-129H
• Product Overview: Recombinant Human AKR1A1(1-325aa) fused with His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-325aa
• Description: Alcohol dehydrogenase [NADP+] (AKR1A1) is a member of the aldo/keto reductase superfamily and catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. This protein is closely related (65% identity) to aldose reductase, an enzyme involved in the pathogenesis of some diabetic and galactosemic complications. It plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Recombinant human Alcohol dehydrogenase was expressed in E. coli and purified by using conventional chromatography techniques.
• Form: Supplied as a liquid in 20mM Tris-HCl (pH8.0)50mM NaCl, 10% glycerol.
• Molecular Mass: 36.5 kDa
• AA Sequence: MAASCVLLHT GQKMPLIGLG TWKSEPGQVK AAVKYALSVG YRHIDCAAIY GNEPEIGEAL KEDVGPGKAV PREELFVTSK LWNTKHHPED VEPALRKTLA DLQLEYLDLY LMHWPYAFER GDNPFPKNAD GTICYDSTHY KETWKALEAL VAKGLVQALG LSNFNSRQID DILSVASVRPAVLQVE CHPY LAQNELIAHC QARGLEVTAY SPLGSSDRAW RDPDEPVLLE EPVVLALAEK YGRSPAQILL RWQVQRKVIC IPKSITPSRI LQNIKVFDFT FSPEEMKQLN ALNKNWRYIV PMLTVDGKRV PRDAGHPLYP FNDPY
• Purity: Affinity Purified~90% (SDS-PAGE) Purified by immunoaffinity chromatography.
• Notes: Small volumes of alcohol dehydrogenase recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
• Storage: Aliquot to avoid repeated freezing and thawing and freeze at -70 centigrade. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Gene Information

• Gene Name: AKR1A1 aldo-keto reductase family 1, member A1 (aldehyde reductase) [ Homo sapiens ]
• Official Symbol: AKR1A1
• Synonyms: AKR1A1; aldo-keto reductase family 1, member A1 (aldehyde reductase); alcohol dehydrogenase [NADP(+)]; ALR; DD3; dihydrodiol dehydrogenase 3; aldehyde reductase; alcohol dehydrogenase; aldo-keto reductase family 1 member A1; ARM; ALDR1; MGC1380; MGC12529
• Gene ID: 10327
• mRNA Refseq: NM_001202414
• Protein Refseq: NP_001189343
• MIM: 103830
• UniProt ID: P14550
• Chromosome Location: 1p33-p32
• Pathway: Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Glycolysis / Gluconeogenesis, organism-specific biosystem; Glycolysis / Gluconeogenesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; tryptophan degradation X (mammalian, via tryptamine), organism-specific biosystem
• Function: L-glucuronate reductase activity; alditol:NADP+ 1-oxidoreductase activity; electron carrier activity; oxidoreductase activity