Recombinant Mouse Atp4a protein, His/GST-tagged

• Cat.No.: Atp4a-20M
• Product Overview: Recombinant Mouse Atp4a(Pro786~Cys1014) fused with His/GST tag at N-terminal was expressed in E. coli.

Specification

• Description: ATPase, H+/K+ exchanging, gastric, alpha polypeptide played an important role in many functions.
• Source: E. coli
• Species: Mouse
• Tag: His/GST
• Form: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Molecular Mass: 58.7kDa
• AA Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- PELTP YLIYITVSVP LPLGCITILF IELCTDIFPS VSLAYEKAES DIMHLRPRNP KRDRLVNEPL AAYSYFQIGA IQSFAGFADY FTAMAQEGWF PLLCVGLRPQ WEDHHLQDLQ DSYGQEWTFG QRLYQQYTCY TVFFISIEMC QIADVLIRKT RRLSVFQQGF FRNKILVIAI VFQVCIGCFL CYCPGMPNIF NFMPIRFQWW LVPMPFGLLI FVYDEIRKLG VRCC
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Atp4a ATPase, H+/K+ exchanging, gastric, alpha polypeptide [ Mus musculus ]
• Official Symbol: Atp4a
• Synonyms: potassium-transporting ATPase alpha chain 1; ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide; H+/K+-ATPase alpha; H+K+-transporting alpha 1; gastric H(+)/K(+) ATPase subunit alpha; proton pump
• Gene ID: 11944
• mRNA Refseq: NM_001290627
• Protein Refseq: NP_001277556
• Chromosome Location: 7 B1; 7 19.2 cM
• Pathway: Collecting duct acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem; Ion transport by P-type ATPases, organism-specific biosystem
• Function: ATP binding; hydrogen:potassium-exchanging ATPase activity; magnesium ion binding