Recombinant Mouse Tnfrsf18 protein, His-tagged Tnfrsf18-15M

Recombinant Mouse Tnfrsf18 protein, His-tagged

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Recombinant Mouse Tnfrsf18 protein, His-tagged

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Cat.No. : Tnfrsf18-15M
Product Overview : Recombinant Mouse Tnfrsf18 protein (Ser22-His153), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 15.2 kDa. The protein migrates as 27-34 kDa under reducing (R) condition (SDS-PAGE).
Protein length : 132
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Tnfrsf18-15M :

Mouse GITR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of Tnfrsf18-15M :

Immobilized Mouse GITR, His Tag at 5 μg/mL (100 μL/well) can bind Mouse GITR Ligand, Fc Tag with a linear range of 5-156 ng/mL (QC tested).

Gene Name : Tnfrsf18
Official Symbol : Tnfrsf18
Synonyms : TNFRSF18; tumor necrosis factor receptor superfamily, member 18; tumor necrosis factor receptor superfamily member 18; glucocorticoid-induced TNFR-related protein; AITR; Gitr
Gene ID : 21936
mRNA Refseq : NM_009400
Protein Refseq : NP_033426
UniProt ID : Q8C4K3

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