"PDIA3" Related Products

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Recombinant Human PDIA3 protein, His-tagged

Cat.No.: PDIA3-20H
Product Overview: Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description: PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol
Bio-activity: Specific activity >20 A650/cm/min/mg. Enzymatic activitywas confirmed by measuring the aggregation of insulin in the presence of DTT.
Molecular Mass: 58.5 kDa (518aa), confirmed by MALDI-TOF
AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEAHSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGKALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL
Purity: >95% by SDS - PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: PDIA3 protein disulfide isomerase family A, member 3 [ Homo sapiens ]
Official Symbol: PDIA3
Synonyms: PDIA3; protein disulfide isomerase family A, member 3; glucose regulated protein, 58kDa , GRP58, protein disulfide isomerase associated 3; protein disulfide-isomerase A3; ERp57; ERp60; ERp61; GRP57; HsT17083; P58; PI PLC; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; ER60; GRP58; PI-PLC;
Gene ID: 2923
mRNA Refseq: NM_005313
Protein Refseq: NP_005304
MIM: 602046
UniProt ID: P30101
Chromosome Location: 15q15
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen Presentation: Folding, assembly and peptide loading of class I MHC, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Asparagine N-linked glycosylation, organism-specific biosystem; Calnexin/calreticulin cycle, organism-specific biosystem;
Function: cysteine-type endopeptidase activity; electron carrier activity; isomerase activity; phospholipase C activity; protein binding; protein disulfide isomerase activity; protein disulfide oxidoreductase activity;

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