Recombinant Human NARS protein, His-tagged

• Cat.No.: NARS-40H
• Product Overview: Recombinant human NARS, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: NARS also known as asparagine--tRNA ligase, cytoplasmic. This protein acts as an enzyme. It is known to catalyze the following reaction: ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH7.4) containing 10% glycerol
• Molecular Mass: 65.3kDa (571aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVLAELY VSDREGSDAT GDGTKEKPFK TGLKALMTVG KEPFPTIYVD SQKENERWNV ISKSQLKNIKKMWHREQMKS ESREKKEAED SLRREKNLEE AKKITIKNDP SLPEPKCVKI GALEGYRGQR VKVFGWVHRL RRQGKNLMFL VLRDGTGYLQ CVLADELCQC YNGVLLSTES SVAVYGMLNL TPKGKQAPGG HELSCDFWEL IGLAPAGGAD NLINEESDVD VQLNNRHMMI RGENMSKILKARSMVTRCFR DHFFDRGYYE VTPPTLVQTQ VEGGATLFKL DYFGEEAFLT QSSQLYLETC LPALGDVFCI AQSYRAEQSR TRRHLAEYTH VEAECPFLTF DDLLNRLEDL VCDVVDRILK SPAGSIVHEL NPNFQPPKRP FKRMNYSDAI VWLKEHDVKK EDGTFYEFGE DIPEAPERLMTDTINEPILL CRFPVEIKSF YMQRCPEDSR LTESVDVLMP NVGEIVGGSM RIFDSEEILA GYKREGIDPT PYYWYTDQRK YGTCPHGGYG LGLERFLTWI LNRYHIRDVC LYPRFVQRCT P
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: NARS asparaginyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: NARS
• Synonyms: NARS; asparaginyl-tRNA synthetase; asparagine--tRNA ligase, cytoplasmic; asparagine tRNA ligase 1; cytoplasmic; NARS1; asparagine tRNA ligase 1, cytoplasmic; asparaginyl-tRNA synthetase, cytoplasmic; ASNRS
• Gene ID: 4677
• mRNA Refseq: NM_004539
• Protein Refseq: NP_004530
• MIM: 108410
• UniProt ID: O43776
• Chromosome Location: 18q21.31
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem; Gene Expression, organism-specific biosystem; tRNA Aminoacylation, organism-specific biosystem
• Function: ATP binding; asparagine-tRNA ligase activity; ligase activity; nucleic acid binding; nucleotide binding