|Cat. No. :
|Product Overview :
||Recombinant Human Interleukin-3 produced inE.Coliis single, a non-glycosylated, Polypeptide chain containing 133 amino acids fragment (20-152) and having a total molecular mass of 15 kDa with an amino-terminal hexahistidine tag. The IL3 His is purified by proprietary chromatographic techniques.
||Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils.
|Physical Appearance :
||Sterile Filtered clear solution.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||Interleukin-3 His is supplied in 1x PBS and 50% glycerol.
|Biological activity :
||The ED50 for this effect is<0.53 ng/ml . measured in a cell proliferation assayusing tf1 human erythroleukemic>0.53>
|Activity Assay :
||1.Cell line: TF1 (human erythroleukemic cell)2.Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF3.Assay Medium: 10% FBS RPMI 1640 without GM-CSF4.Cell Density: 2 x 104 cells/well (96 well plate, final volume 100ul)5.Starvation : 24hr, Assay medium6.Incubation Time : 48 hr (after sample treatment)7.Concentration Range: 0.048ng/ml - 25ng/ml 8.Detection method : MTT assay
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.