Recombinant Human IL3, His-tagged
Cat.No. : | IL3-56H |
Product Overview : | Recombinant Human Interleukin-3 produced inE.Coliis single, a non-glycosylated, Polypeptide chain containing 133 amino acids fragment (20-152) and having a total molecular mass of 15 kDa with an amino-terminal hexahistidine tag. The IL3 His is purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | IL3-56H |
Description : | Interleukin-3 is a pleiotropic cytokine produced primarily by activated T cells. IL-3 is thought to function via specific cell surface receptors to stimulate the proliferation, differentiation and survival of haematopoietic cell lines. IL-3 has also been shown to affect the functional activity of a variety of other cell types including mast cells, eosinophils, megakaryocytes and basophils. |
Source : | Escherichia Coli. |
Physical Appearance : | Sterile Filtered clear solution. |
Purity : | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : | Interleukin-3 His is supplied in 1x PBS and 50% glycerol. |
Biological activity : | The ED50 for this effect is<0.53 ng/ml . measured in a cell proliferation assayusing tf1 human erythroleukemic>0.53> |
Activity Assay : | 1.Cell line: TF1 (human erythroleukemic cell)2.Maintenance Condition: 10% FBS RPMI 1640 with GM-CSF3.Assay Medium: 10% FBS RPMI 1640 without GM-CSF4.Cell Density: 2 x 104 cells/well (96 well plate, final volume 100ul)5.Starvation : 24hr, Assay medium6.Incubation Time : 48 hr (after sample treatment)7.Concentration Range: 0.048ng/ml - 25ng/ml 8.Detection method : MTT assay |
Storage : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. |
Gene Name : | IL3 interleukin 3 (colony-stimulating factor, multiple) [ Homo sapiens ] |
Synonyms : | IL3; interleukin 3 (colony-stimulating factor, multiple); IL-3; MCGF; MGC79398; MGC79399; MULTI-CSF; interleukin 3; mast-cell growth factor; P-cell stimulating factor; hematopoietic growth factor; Mast cell growth factor; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; Hematopoietic growth factor |
Gene ID : | 3562 |
mRNA Refseq : | NM_000588 |
Protein Refseq : | NP_000579 |
MIM : | 147740 |
UniProt ID : | P08700 |
Chromosome Location : | 5q31.1 |
Pathway : | Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; Apoptosis; Asthma |
Function : | cytokine activity; growth factor activity; protein binding; interleukin-3 receptor binding |
Products Types
◆ Recombinant Protein | ||
Il3-027I | Active Recombinant Mouse Il3 Protein (135 aa) | +Inquiry |
IL3-168H | Active Recombinant Human IL3 Protein | +Inquiry |
IL3-169M | Active Recombinant Mouse IL3 Protein | +Inquiry |
IL3-436H | Active Recombinant Human IL3 protein(Ala20-Phe152) | +Inquiry |
Il3-247I | Active Recombinant Rat Il3 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
IL3-445RCL | Recombinant Rat IL3 cell lysate | +Inquiry |
IL3-607HCL | Recombinant Human IL3 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (16)
Ask a questionIn vitro, IL-3 can induce differentiation and proliferation of myeloid lineage cells and is distinguished from GM-CSF by the relative increase in production of platelets, basophils, and mast cells.
Although effective, IL-3 appears to present no therapeutic advantages compared to G-CSF and GM-CSF, and in direct comparison studies, IL-3 led to a considerably higher rate of adverse events than either of the other cytokines.
mice deficient in both bc and IL-3 show the same phenotype as the bc-deficient mice, conclusively demonstrating that, for the most part, the GM-CSF/IL3/IL5 family is not essential for steady-state production of myeloid cells
Unlike in humans, murine IL-3 has a second b chain receptor, bcIL3, which bears 91% homology to the bc chain.
Knockout mice were also reported with surprisingly limited phenotypes. Despite the ability of the three cytokines to stimulate hematopoiesis, mice functionally deficient in all three cytokinesdisplay normal circulating levels of granulocytes, monocytes, and basophils.
non-hematopoietic (e.g., epithelial cells, and fibroblasts) cell subsets can also produce il-3.
Such as allergic inflammation and parasitic infestation.
IL-3 can regulate the survival of basophil granulocytes and cytokine production through autocrine, so as to achieve the purpose of treating allergic diseases.
Bhlhe40
E.g., lymphocytes,epithelial cells, and fibroblasts
Yes, there are some completed clinical trials on IL3 results, but the specific results need to refer to the relevant research papers.
This protein can promote stem cell proliferation and differentiation during hematopoietic process, thus increasing the production of different types of blood cells.
In general, the IL3 has relatively few side effects, but some unpleasant symptoms such as fever, headache and nausea may occur.
IL3 can regulate the body's immune response and the function of immune cells, and plays an important role in immune regulation.
IL3 generally have good solubility and stability, making them easier to handle during drug preparation and storage.
The study of IL3 is ongoing, including its application in the development of therapeutic drugs, as well as in combination therapy with other immune molecules.
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