|Cat. No. :
|Product Overview :
||Recombinant Human intracisternal A particle-promoted polypeptide produced inE.Coliis a single, non-glycosylated polypeptide chain. The protein contains 157 amino acids and has a molecular mass of 17.3 kDa.
||Intracisternal A particle-promoted polypeptide(IPP) is a 66kDa protein(584 amino acids), which contains an N-terminal POZ protein-protein interaction domain and a C-terminla kelch repeat domain consisting of six tandem arranged repeats. The POZ domain(also called BTB domain) is present near the N-terminus of a fraction of zinc finger proteins and in protein that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes including N-coR and SMRT.
|Amino Acid Sequence :
||MANEDCPKAA DSPFSSDKHA QLILAQINKM RNGQHFCDVQ LQVGQESFKA HRLVLAASSPYFAALFTGGM KESSKDVVPI LGIEAGIFQI LLDFIYTGIV NIGV NNVQEL IIAADMLQLTEVVHLCCEFL KGQIDPLNCI GIFQFSEQIA CHDLLEF.
|Physical Appearance :
||Sterile filtered clorless solution.
||The protein (1mg/ml) containing 10mM HEPES (pH7.4) and 25mM NaCl.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).