||Recombinant human S100A4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 13.8 kDa (121aa).
||S100A4, also known as S100 calcium-binding protein A4, belongs to the S100 super-family of proteins containing 2 EF-hand calcium binding domains. This protein is ubiquitously overexpressed and is localized in the cytoplasm and/or nucleus. S100A4 may play a role in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of the S100A4 gene have been implicated in tumor metastasis.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay).
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MACPLEKALD VMVSTFHKYSGKEGDKFKLN KSELKELLTR ELPSFLGKRT DEAAFQKLMS NLDSNRDNEVDFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.