||Recombinant Human A Parkinson's disease-related point mutant (A53T) of α-Synuclein. A53T Mutant of α-Synuclein was overexpressed inE.coliand the recombinant protein was purified to apparent homogeneity by taking advantage of the thermo solubility of the protein and by using conventional column chromatography techniques.
||Alpha-synuclein also known as SNCA is a protein which in humans is encoded by the SNCA gene. An alpha-synuclein fragment, known as the non-Abeta component (NAC) of Alzheimer"s disease amyloid, originally found in an amyloid-enriched fraction, is shown to be a fragment of its precursor protein, NACP, by cloning of the full-length cDNA. Later, NACP turned out to be a human homologue of Torpedo synuclein. Therefore, NACP is also referred to as human alpha-synuclein.
||Sterile filtered clorless solution.
||Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.
||The protein (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl .
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).