Recombinant Human Thioredoxin

• Cat.No.: TXN-97H
• Product Overview: Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques (105 aa, 11.7 kDa).

Specification

• Cat. No.: TXN-97H
• Description: Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
• Source: Escherichia Coli.
• Formulation: Liquid. In PBS (pH 7.4).
• Molecular Weight: 11.7 kDa (105 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Amino acid Sequence: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV
• Biological activity: Specific activity is 7-10 A650/min/mg and was obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. Please refer to our activity assay protocol.
• Activity Assay: 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.
• Storageee: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TXN thioredoxin [ Homo sapiens ]
• Synonyms: TXN; MGC61975; TRX; ADF; DKFZp686B1993; MGC61975; OTTHUMP00000021892; SASP; TRDX; TRX1; Trx; thioredoxin; ATL-derived factor; Surface-associated sulphydryl protein.
• Gene ID: 7295
• mRNA Refseq: NM_003329
• Protein Refseq: NP_003320
• MIM: 187700
• UniProt ID: P10599
• Chromosome Location: 9q31
• Pathway: Nucleotide metabolism.
• Function: protein binding.