Active Recombinant Listeriolysin O, PEST Free
| Cat.No. : | hly-243 |
| Product Overview : | Recombinant Listeriolysin O lacking both the signal secretion sequence and the PEST- like sequence is produced in E.Coli. LLO-PEST minus is composed of 471(60-592) amino acids, with the addition of a methionine in its NH2-terminus. |
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| Cat. No. : | hly-243 |
| Description : | The secreted pore-forming protein listeriolysin O (LLO) is an essential virulence determinant of the facultative intracellular bacterium Listeria monocytogenes that allows the pathogen to escape from the phagocytic vacuole and reach the host cytosol. LLO is a single polypeptide protein encoded by the hlyA gene and composed of 529 residues. At its NH2-terminus it possesses a 25 residues long typical signal sequence excited during the secretion process. Moreover, in its NH2-terminus there is also a 19 amino acids PEST- like sequence that may target this toxin for degradation. This motif seems to be essential for bacterial virulence too, since its deletion causes a defect in bacterium phagosomal escaping. |
| Source : | Escherichia Coli. |
| Physical Appearance : | Sterile Filtered clear solution. |
| Formulation : | The protein (0.2mg/ml) contains 50mM NaH2PO4, 1mM EDTA, 2.7mM KCl, 1mM DTT, 5% glycerol and 0.5M NaCl. |
| Purity : | Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Activity : | 1.25x106HU/mg. 2 mM DTT could be use to reactivate the toxin. |
| Endotoxin : | Less than 0.1ng/µg (IEU/µg) of LLO. |
| Applications : | a. Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA (4). b. Production of specific monoclonal antibodies (5). c. Detection of anti-listeriolysin O antibodies (6). |
| Stability : | rLLO although stable at 14C for 1 week, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles. |
| Gene Name : | hly listeriolysin O [ Listeria monocytogenes str. 4b F2365 ] |
| Synonyms : | Hly; listeriolysin O; identified by similarity to SP:P13128; match to protein family HMM PF01289 |
| Gene ID : | 2797098 |
| Protein Refseq : | YP_012823 |
| UniProt ID : | Q724L1 |
| Function : | cholesterol binding |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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