||Recombinant Rat PV protein produced inE. Colicontains full length amino acids and has a molecular mass of 9-11 kDa.
||Parvalbumin is a calcium binding albumin protein with low molecular weight (typically 9-11 kDa). It has three EF hand motifs and is structurally related to calmodulin and troponin C. Parvalbumin is localised in fast-contracting muscles, where its levels are highest, and in the brain and some endocrine tissues.
||Sterile Filtered White lyophilized (freeze-dried) powder.
||The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-base, 70mM NaCl, 1mM EDTA and 1mM -mercaptoethanol, pH 7.4.
||Greater than 90% as determined by: Analysis by reducing and non-reducing SDS-PAGE Coomassie.
||It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: A- Dilute 1 µl of the mon oclonal antibody 235 (Swant) against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5"000). B- Add 1µg of the Parvalbumin to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.
||Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Parvalbumin should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.