||Recombinant Vim protein produced inE. Colihas a molecular weight of 53,685 dalton (calaulated from sequence), and 57,000 dalton (determinated by SDS gelelctrophoresis).
||Vimentin is a member of the intermediate filament family of proteins. Intermediate filaments are an important structural feature of eukaryotic cells. They, along with microtubules and actin microfilaments, make up the cytoskeleton. Although most intermediate filaments are stable structures, in fibroblasts, vimentin exists as a dynamic structure. Interestingly, vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.
||Sterile Filtered White lyophilized (freeze-dried) powder
||The protein (1mg/ml) was lyophilized with 30mM Tris/HCL, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride.
||Greater than 95.0% as determined by: (a) SDS-gelelctrophoresis.
||It is recommended to reconstitute the rHuVim in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized rHuVim although stable at room temperature for 3 weeks, should be stored desiccated below-18℃. Upon reconstitution rHuVim should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.