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Recombinant Yeast LMA1

Cat. No. : LMA1-98Y
Product Overview : Recombinant yeast Thioredoxin produced inE.Coliis a single, non-glycosylated, polypeptide chain and has a molecular mass of 12.6kDa.
Description : Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance.
Source : Escherichia Coli.
Physical Appearance : Sterile Lyophilized Powder.
Formulation : Each mg of protein contains 20mM phosphate buffer pH 7.4.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
Solubility : It is recommended to reconstitute the lyophilized ryTRX in sterile 18MΩ-cm H2O.
Dimers And Aggregates : Less than 1% as determined by silver stained SDS-PAGE gel analysis.
Endotoxin : Less than 0.1 ng/µg (IEU/µg) of ryTRX.
Biological Activity : RyTRX is fully biologically active when compared to standard. TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5). The specific activity was found to be 3IU/mg.
Stability : RyTRX although stable at 4°Cfor 3 weeks, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.

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