Recombinant Yeast LMA1

• Cat.No.: LMA1-98Y
• Product Overview: Recombinant yeast Thioredoxin produced inE.Coliis a single, non-glycosylated, polypeptide chain and has a molecular mass of 12.6kDa.

Specification

• Species: Yeast
• Source: E.coli
• Tag: Non
• Description: Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance.
• Physical Appearance: Sterile Lyophilized Powder.
• Formulation: Each mg of protein contains 20mM phosphate buffer pH 7.4.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.
• Solubility: It is recommended to reconstitute the lyophilized ryTRX in sterile 18MΩ-cm H2O.
• Dimers And Aggregates: Less than 1% as determined by silver stained SDS-PAGE gel analysis.
• Endotoxin: Less than 0.1 ng/µg (IEU/µg) of ryTRX.
• Biological Activity: RyTRX is fully biologically active when compared to standard. TRX activity is assayed by measuring the change in absorbance at 650 nm at 25°C using 0.13µM bovine insulin containing 0.33mM DTT (pH 6.5). The specific activity was found to be 3IU/mg.
• Stability: RyTRX although stable at 4°Cfor 3 weeks, should be stored desiccated below -18°C. Please prevent freeze thaw cycles.

Gene Information

• Gene Name: TRX1 Cytoplasmic thioredoxin isoenzyme of the thioredoxin system which protects cells against oxidative and reductive stress, forms LMA1 complex with Pbi2p, acts as a cofactor for Tsa1p, required for ER-Golgi transport and vacuole inheritance [ Saccharomyces cerevisiae ]
• Synonyms: LMA1; Thioredoxin; TRX1; YLR043C
• Gene ID: 850732
• Protein Refseq: NP_013144
• UniProt ID: TXBY2
• Chromosome Location: XII