Recombinant Human Agrin, His-tagged


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Recombinant Human Agrin, His-tagged

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Cat. No. : AGRN-131H
Product Overview : Recombinant Human agrin antigen (His-tagged) expressed inSf9has a molecular mass of 43,591 Dalton. The isoelectric point of the antigen is pH 8.9.
Description : Human α3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Source : Sf9 insect cells.
Coating Concentration : 0.12-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Applications : Standard ELISA test; Immuno-Dot test with positive/negative sera panels; SDS-PAGE; Western-Blot.
Caution : It has been reported that the immunodominant epitope of GBM is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
Storage & Stability : Recommendations for storage buffer: ion strength 100mM, neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent. Storage temperature: –70° to –80° C. Please prevent freeze-thaw cycles.
Gene Name : AGRN agrin [ Homo sapiens ]
Synonyms : AGRN; agrin; FLJ45064; agrin proteoglycan; Agrin;AGRIN
Gene ID : 375790
mRNA Refseq : NM_198576
Protein Refseq : NP_940978
MIM : 103320
UniProt ID : O00468
Chromosome Location : 1p36.33
Pathway : ECM-receptor interaction; Axon guidance
Function : acetylcholine receptor regulator activity; laminin binding; structural constituent of cytoskeleton
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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