||Recombinant Human agrin antigen (His-tagged) expressed inSf9has a molecular mass of 43,591 Dalton. The isoelectric point of the antigen is pH 8.9.
||Human α3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
||Sf9 insect cells.
||0.12-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
||Standard ELISA test; Immuno-Dot test with positive/negative sera panels; SDS-PAGE; Western-Blot.
||It has been reported that the immunodominant epitope of GBM is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
|Storage & Stability:
||Recommendations for storage buffer: ion strength 100mM, neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent. Storage temperature: –70° to –80° C. Please prevent freeze-thaw cycles.