Recombinant Human Agrin, His-tagged
Cat.No. : | AGRN-131H |
Product Overview : | Recombinant Human agrin antigen (His-tagged) expressed inSf9has a molecular mass of 43,591 Dalton. The isoelectric point of the antigen is pH 8.9. |
- Specification
- Gene Information
- Related Products
- Download
Cat. No. : | AGRN-131H |
Description : | Human α3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen. |
Source : | Sf9 insect cells. |
Coating Concentration : | 0.12-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |
Applications : | Standard ELISA test; Immuno-Dot test with positive/negative sera panels; SDS-PAGE; Western-Blot. |
Caution : | It has been reported that the immunodominant epitope of GBM is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298). |
Storage & Stability : | Recommendations for storage buffer: ion strength 100mM, neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent. Storage temperature: –70° to –80° C. Please prevent freeze-thaw cycles. |
Gene Name : | AGRN agrin [ Homo sapiens ] |
Synonyms : | AGRN; agrin; FLJ45064; agrin proteoglycan; Agrin;AGRIN |
Gene ID : | 375790 |
mRNA Refseq : | NM_198576 |
Protein Refseq : | NP_940978 |
MIM : | 103320 |
UniProt ID : | O00468 |
Chromosome Location : | 1p36.33 |
Pathway : | ECM-receptor interaction; Axon guidance |
Function : | acetylcholine receptor regulator activity; laminin binding; structural constituent of cytoskeleton |
Products Types
◆ Recombinant Protein | ||
AGRN-2443H | Recombinant Human AGRN Protein, His (Fc)-Avi-tagged | +Inquiry |
AGRN-220R | Recombinant Rat AGRN Protein, His (Fc)-Avi-tagged | +Inquiry |
Agrn-1789R | Recombinant Rat Agrin | +Inquiry |
Agrn-6783M | Recombinant Mouse Agrn protein, His & T7-tagged | +Inquiry |
Agrn-6784R | Recombinant Rat Agrn protein, His & T7-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (10)
Ask a questionThe measurement of AGRN levels or its isoforms in biological fluids or tissues may hold potential as diagnostic markers for certain neurological disorders, although further research is needed.
AGRN interacts with specific receptors on the muscle and nerve cells, initiating a signaling cascade that leads to the clustering of acetylcholine receptors and the formation and maintenance of the NMJ.
Yes, AGRN has been shown to have roles outside of the NMJ, including in the development of the central nervous system, kidneys, and cardiovascular system.
AGRN exists in multiple isoforms generated by alternative splicing. The most well-known isoforms include the neural isoforms (Agrin-N) and the muscle-specific isoforms (Agrin-M).
AGRN consists of several functional domains, including N-terminal laminin G-like (LG) domains, central EGF-like repeats, a C-terminal domain, and a heparin-binding site.
AGRN has been found to promote tumor cell migration, invasion, and metastasis by interacting with receptors and signaling pathways that regulate cell adhesion, migration, and angiogenesis.
AGRN expression is tightly regulated during development and can be influenced by various signaling pathways, including Wnt, BMP, and Notch signaling.
AGRN interacts with two major receptors: muscle-specific kinase (MuSK) on the postsynaptic membrane and low-density lipoprotein receptor-related protein 4 (LRP4) on the presynaptic membrane.
AGRN plays a role in regulating synaptic plasticity by modulating the structure and function of synapses, including their strength and stability.
AGRN regulates the maturation and stabilization of synapses by promoting the clustering of postsynaptic receptors and facilitating the development of pre- and postsynaptic specializations.
Customer Reviews (5)
Write a reviewThe packaging was secure, and the proteins maintained their integrity and activity upon arrival.
I highly recommend their products for researchers seeking reliable reagents.
They offer excellent value for the price.
We rely on this protein for its consistent performance.
I am extremely satisfied with the prompt delivery of the proteins.
Ask a Question for All AGRN Products
Required fields are marked with *
My Review for All AGRN Products
Required fields are marked with *
Inquiry Basket