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Recombinant Human Agrin, His-tagged

Cat. No.: AGRN-131H
Product Overview: Recombinant Human agrin antigen (His-tagged) expressed inSf9has a molecular mass of 43,591 Dalton. The isoelectric point of the antigen is pH 8.9.
Description: Human α3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Binds IgG-type human auto-antibodies. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Source: Sf9 insect cells.
Coating Concentration: 0.12-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Applications: Standard ELISA test; Immuno-Dot test with positive/negative sera panels; SDS-PAGE; Western-Blot.
Caution: It has been reported that the immunodominant epitope of GBM is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298).
Storage & Stability: Recommendations for storage buffer: ion strength 100mM, neutral to slightly alkaline pH and 20 % glycerol as cryoprotective agent. Storage temperature: –70° to –80° C. Please prevent freeze-thaw cycles.
Gene Name: AGRN agrin [ Homo sapiens ]
Synonyms: AGRN; agrin; FLJ45064; agrin proteoglycan; Agrin;AGRIN
Gene ID: 375790
mRNA Refseq: NM_198576
Protein Refseq: NP_940978
MIM: 103320
UniProt ID: O00468
Chromosome Location: 1p36.33
Pathway: ECM-receptor interaction; Axon guidance
Function: acetylcholine receptor regulator activity; laminin binding; structural constituent of cytoskeleton

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