Active Recombinant Human Tumor Protein P53

• Cat.No.: TP53-170H
• Product Overview: Recombinant Human TP53 protein expressed inE.coliencodesfull length amino acids and has a molecular mass of 43.7 kDa.

Specification

• Cat. No.: TP53-170H
• Description: p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
• Source: E.coli.
• Formulation: 5µg/5µl purified human p53 in 20mM Tris-Acetate, pH 8, 0.6M Nacl and 50% Glycerol.
• Application: • P53 is an excellent substrate for kinase assays; • It can be used as a reagent for development of DNA based binding Assay.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Activity: Active in DNA binding assays.
• Storage & Stability: For long term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: TP53 tumor protein p53 [ Homo sapiens ]
• Synonyms: TP53; tumor protein p53; p53; LFS1; TRP53; FLJ92943; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; p53 transformation suppressor; p53 tumor suppressor; transformation-related protein 53
• Gene ID: 7157
• mRNA Refseq: NM_000546
• Protein Refseq: NP_000537
• MIM: 191170
• UniProt ID: P04637
• Chromosome Location: 17p13.1
• Pathway: Amyotrophic lateral sclerosis (ALS); Apoptosis; Basal cell carcinoma; Bladder cancer; Cell cycle; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; Glioma; Huntington"s disease; MAPK signaling pathway; Melanoma; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Small cell lung cancer; Thyroid cancer; Wnt signaling pathway; p53 signaling pathway; Cell Cycle Checkpoints
• Function: ATP binding; DNA strand annealing activity; chaperone binding; chromatin binding; copper ion binding; enzyme binding; metal ion binding; promoter binding; protein N-terminus binding; protein heterodimerization activity; transcription factor activity; transcription factor binding; zinc ion binding