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Active Recombinant Human Amphiregulin

Cat.No. : AREG-134H
Product Overview : Recombinant Human Amphiregulin encoding the human Amphiregulin protein sequence (containing the signal peptide, N- and C-terminal propeptide and the mature Amphiregulin sequence) was expressed in modifiedhuman 293 cells. Proteolytic processing of the expressed membrane-bound precursor protein generates mature soluble Amphiregulin.
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  • Gene Information
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Cat. No. : AREG-134H
Description : Amphiregulin is an epidermal growth factor (EGF)-related glycoprotein that is expressed in a variety of tissues including ovary, placenta, lung, kidney, stomach, colon and breast. As an EGF-related growth factor, Amphiregulin is involved in the differentiation and proliferation of a wide range of cell types and these actions are mediated through binding to the EGF receptor.
Source : Human 293 cells.
Amino Acid Sequence : VVKPPQNKTESENTSDKPKRKKKGG KNGKNRRNRKKKNPCNAEFQNFCIH GECKYIEHLEAVTCKCQQEYFGERC GEKSMK.
Molecular Mass : Under reducing conditions Amphiregulin (mature form) migrates as a broad band between 17.6 and 23.4 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified Amphiregulin polypeptide that has a predicted monomeric molecular mass of 9.07 kDa.
% Carbohydrate : Purified Amphiregulin (mature form) consists of 25–50% carbohydrate by weight.
Glycosylation : Amphiregulin (mature form) contains N- and O-linked oligosaccharides.
Purity : >95%, as determined by SDS-PAGE, visualized by Coomassie Brilliant Blue.
Formulation : When reconstituted in 0.5 ml sterile 5mM acetic acid, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reconstitution : It is recommended that 0.5 ml of sterile 5mM acetic acid be added to the vial.
Storage : Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, with longer-term storage in aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
Activity : The ED50of Amphiregulin (mature form) is typically 10–15 ng/ml as measured in a cell proliferation assay using the murine Balb/3T3 fibroblast cell line.
Publication :
Epidermal Growth Factor Receptor (EGFR) Signaling Is a Key Mediator of Hormone-Induced Leukocyte Infiltration in the Pubertal Female Mammary Gland (2014)
Gene Name : EG amphiregulin [ Homo sapiens ]
Synonyms : AREG; amphiregulin; AR; SDGF; CRDGF; MGC13647; schwannoma-derived growth factor; colorectum cell-derived growth factor; OTTHUMP00000160473
Gene ID : 374
mRNA Refseq : NM_001657
Protein Refseq : NP_001648
UniProt ID : P15514
Chromosome Location : 4q13-q21
MIM : 104640
Pathway : ErbB signaling pathway
Function : cytokine activity; growth factor activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (34)

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Does AREG play a role in inflammatory diseases? 02/18/2023

Yes, AREG is implicated in various inflammatory diseases, such as asthma and inflammatory bowel disease, where it can modulate immune responses and contribute to tissue inflammation.

How is the AREG protein released from cells? 10/16/2022

AREG can be released from cells through multiple mechanisms, such as proteolytic cleavage by metalloproteases, exocytosis, and shedding of its precursor form.

Are there any diseases associated with dysregulation of AREG? 08/25/2022

Yes, dysregulation of AREG has been linked to various diseases, including cancer, inflammatory disorders, and certain gastrointestinal conditions.

How does AREG contribute to wound healing? 07/23/2022

AREG plays a critical role in wound healing by promoting cell migration, proliferation, and tissue regeneration, particularly in epithelial tissues.

Can AREG be used as a therapeutic target in cancer treatment? 12/22/2021

AREG has been identified as a potential therapeutic target in cancer treatment due to its involvement in promoting cancer cell growth and survival. However, further research is needed to explore its clinical utility.

What are the effects of AREG on cancer cell proliferation? 08/12/2021

AREG can promote cancer cell proliferation by binding to the EGFR and activating downstream signaling pathways that drive cell cycle progression and survival.

Is AREG involved in angiogenesis? 05/26/2021

Yes, AREG has been shown to promote angiogenesis, the formation of new blood vessels, by stimulating endothelial cell proliferation and migration.

Does AREG have any role in neurodegenerative diseases? 03/03/2021

There is limited research suggesting that AREG may have a role in neurodegenerative diseases. Some studies have shown altered AREG expression in conditions like Alzheimer's disease and Parkinson's disease, but more research is needed to fully understand its significance in these diseases.

Are there any side effects associated with blocking AREG signaling? 02/10/2021

Blocking AREG signaling may lead to potential side effects, as AREG is involved in various physiological processes. However, the specific side effects would depend on the context and the therapeutic approach used.

Are there any AREG-targeted therapies currently available? 12/27/2020

Currently, there are no approved therapies specifically targeting AREG, but there are ongoing clinical trials investigating the efficacy of targeting AREG and its associated pathways in different diseases.

Are there any ongoing clinical trials targeting AREG? 12/19/2020

There are ongoing clinical trials investigating the potential use of inhibitors and antibodies targeting AREG in cancer treatment. These trials aim to evaluate the safety and efficacy of AREG-targeted therapies alone or in combination with other treatments.

Does AREG have any role in the nervous system? 08/01/2020

Yes, AREG is implicated in various neurological processes, including neuroprotection, neural development, and synaptic plasticity.

Can AREG be produced by other cell types besides cancer cells? 07/24/2020

Yes, AREG can be produced by various cell types, including epithelial cells, fibroblasts, immune cells, and endothelial cells. Its production can be stimulated by different factors depending on the cell type and the physiological context.

How is AREG involved in mammary gland development and lactation? 01/26/2020

AREG plays a crucial role in mammary gland development and lactation by promoting the growth and differentiation of mammary epithelial cells, as well as stimulating milk production.

Is the expression of AREG protein influenced by hormones? 01/10/2020

Yes, the expression of AREG can be regulated by hormones, such as estrogens and progesterone, in various target tissues.

Can AREG be used as a biomarker for any diseases? 09/23/2019

AREG expression levels have been investigated as potential biomarkers for certain cancers, including colorectal, breast, and ovarian cancer, as well as inflammatory bowel disease.

Does AREG have any effects on cell differentiation? 09/23/2019

Yes, AREG can influence cell differentiation in certain contexts. It has been shown to promote the differentiation of various cell types, including mammary epithelial cells, neuronal progenitor cells, and keratinocytes.

Is there any evidence of AREG being involved in metastasis? 08/22/2019

There is evidence suggesting that AREG can promote metastasis in certain types of cancer. It may contribute to the invasive and migratory properties of cancer cells, aiding their spread to distant organs.

How is the AREG protein activated? 05/29/2019

The AREG protein can be activated by various signals, including growth factors, cytokines, and cell-cell interactions.

Is there any research on AREG protein in other fields apart from cancer? 02/19/2019

Yes, AREG protein research is also emerging in fields such as reproductive biology, neurological disorders, and skin diseases.

Does AREG have any role in immune response modulation? 01/31/2019

Yes, AREG has immunomodulatory properties and can influence immune cell functions, such as regulating T cell differentiation and function, and influencing the balance between immune activation and tolerance.

Can AREG protein be used as a therapeutic target? 01/16/2019

Yes, targeting AREG and its signaling pathways has shown promise in developing potential therapeutic interventions against cancer and other diseases.

Can AREG be used in targeted therapy? 12/07/2018

AREG has the potential to be used in targeted therapy, especially in cancers where AREG signaling is implicated in promoting tumor growth and survival. Targeting AREG or its signaling pathways could potentially be used in combination with other therapies to improve treatment outcomes.

Are there any inhibitors or antibodies targeting AREG? 06/28/2018

Yes, there are experimental inhibitors and anti-AREG antibodies being studied for their potential as therapeutic agents in various diseases, including cancer.

Is AREG involved in wound healing? 03/06/2018

Yes, AREG has been shown to play a role in wound healing. It stimulates the migration and proliferation of cells involved in the wound healing process, such as fibroblasts and endothelial cells, promoting tissue repair.

Can AREG be used as a diagnostic marker for cancer? 01/24/2018

AREG expression levels have been evaluated as potential diagnostic markers for certain types of cancer, including colorectal and breast cancer, to predict prognosis and guide treatment decisions.

What other cellular processes are regulated by AREG? 12/12/2017

In addition to its involvement in cellular proliferation, tissue development, and immune responses, AREG also influences processes such as cell migration, apoptosis, and stem cell maintenance.

Has the AREG gene been linked to any genetic disorders? 09/27/2017

There is limited evidence suggesting that genetic variations in the AREG gene may be associated with an increased risk of certain disorders, such as inflammatory diseases and certain types of cancers. However, more research is needed to fully understand the genetic implications of AREG.

Can AREG be used in regenerative medicine? 03/24/2017

AREG has shown promise in regenerative medicine due to its ability to stimulate tissue regeneration and repair. Further research is needed to fully understand its potential in this field.

Is AREG protein involved in normal physiological processes? 03/05/2017

Yes, AREG protein is involved in normal physiological processes such as tissue development, wound healing, and immune response regulation.

Can AREG be used as a prognostic marker for cancer? 01/26/2017

AREG expression levels have been evaluated as potential prognostic markers in several types of cancer. High AREG expression has been associated with poorer outcomes in some cases. However, further studies are needed to validate its clinical utility as a prognostic marker.

Are there any genetic mutations associated with AREG protein? 09/10/2016

Yes, certain genetic mutations in the AREG gene have been identified and linked to specific disorders, including colorectal cancer and asthma.

What are the downstream signaling pathways activated by AREG? 03/06/2016

AREG can activate various signaling pathways, including the EGFR (epidermal growth factor receptor) pathway, MAPK (mitogen-activated protein kinase) pathway, and PI3K (phosphoinositide 3-kinase)/Akt pathway.

Are there any inhibitors or antibodies targeting AREG currently in development? 02/24/2016

There are ongoing efforts to develop inhibitors and antibodies targeting AREG for various therapeutic purposes, including cancer treatment. Some preclinical and early-stage clinical trials have been conducted, but more research is needed before these agents can be widely used in clinical practice.

Customer Reviews (8)

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Reviews
08/17/2022

    The high-quality AREG protein available from reliable manufacturers ensures accurate detection and quantification of ANO4 protein levels in samples, providing valuable insights into its biological roles and signaling pathways.

    10/26/2020

      In WB experiments, AREG protein exhibits exceptional performance, making it an ideal tool for studying ANO4 protein expression, regulation, and potential functional changes.

      09/04/2020

        AREG protein demonstrates great utility in protein electron microscopy structure analysis.

        11/05/2019

          Its remarkable specificity and functionality have allowed me to investigate various cellular processes with precision and confidence.

          03/02/2019

            the manufacturer's support has been instrumental in assisting me throughout my work with the AREG protein.

            01/18/2018

              Its exceptional quality ensures accurate data and supports the validity of my research findings.

              05/22/2017

                the high-quality AREG protein is available in various forms, including recombinant proteins and purified samples, enabling researchers to select the most suitable format for their specific experimental setup.

                03/05/2017

                  The technical team's expertise and promptness in addressing any queries or concerns have been extraordinary.

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