| Cat. No. : |
NGFR-453H |
| Description : |
Nerve growth factor receptor (NGFR), also known as p75NTR or CD271 antigen, is a low affinity NGF receptor primarily responsible for modulating the affinity and activity of tyrosine kinases that promote neuronal survival. Ligand binding by neurotrophins, including beta NGF, brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT3) and neurotrophin-5 (NT4/5), to the NGF R can promote either survival or apoptosis of neurons. NGF receptors such as TrkA, B and C, can associate with NGF R to stimulate higher affinity ligand binding, although on its own NGF R binds to all neutrophins within equal affinity. Neurotrophin-NGFR signaling mediates conditions such as pain, depression, obesity, nerve regeneration disorders, learning and memory. Additionally, NGF R is thought to play a role in neuronal death that occurs in disorders of the CNS such as Alzheimer's disease. |
| Source : |
human 293 cells. |
| Molecular Mass : |
NGFR(222aa) - Fc Chimera migrates as a broad band between 75 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified NGFR(222aa) – Fc Chimera that has a predicted molecular mass of 50.8 kDa. |
| pI : |
NGFR(222aa) - Fc Chimera separates into a number of isoforms with a pI between 4.0 and 6.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified NGFR(222aa) – Fc Chimera that has a predicted pI of 4.89. |
| % Carbohydrate : |
NGFR (222aa) - Fc Chimera consists of 30-50% carbohydrate by weight. |
| Glycosylation : |
NGFR (222aa) - Fc Chimera contains N-linked and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. |
