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Recombinant Human Peroxiredoxin 6, His-tagged

Cat. No.: PRDX6-188H
Product Overview: Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 27.1 kDa (244aa).
Description: Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Source: E.coli.
Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP.
Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% Glycerol.
Purity: > 95% by SDS – PAGE.
Concentration: 1 mg/ml (determined by Bradford assay).
Biological activity: Specific activity: approximately 95-120 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
Activity Assay: 1. Prepare a 50ul reaction mix into a suitable container: the final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2. Add 5 ul of recombinant PRDX6 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3. Incubate at 25°C for 2 minutes. 4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) 7. Record the increase in A475nm.
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: PRDX6 peroxiredoxin 6 [ Homo sapiens ]
Synonyms: PRDX6; peroxiredoxin 6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; KIAA0106; MGC46173; 1-Cys peroxiredoxin; antioxidant protein 2; non-selenium glutathione peroxidase; acidic calcium-independent phospholipase A2; EC 1.11.1.15; EC 1.11.1.7; p29; Antioxidant protein 2; 1-Cys PRX; EC 3.1.1.-; 24 kDa protein; Liver 2D page spot 40; Red blood cells page spot 12; OTTHUMP00000032693; 1-Cys peroxiredoxin; Non-selenium glutathione peroxidase
Gene ID: 9588
mRNA Refseq: NM_004905
Protein Refseq: NP_004896
MIM: 602316
UniProt ID: P30041
Chromosome Location: 1q24.1
Pathway: Biosynthesis of phenylpropanoids; Metabolic pathways; Methane metabolism; Phenylalanine metabolism; Tropane, piperidine and pyridine alkaloid biosynthesis
Function: glutathione peroxidase activity; hydrolase activity; oxidoreductase activity; peroxiredoxin activity; phospholipase A2 activity

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