| Cat. No. : |
WISP3-184H |
| Description : |
WISP-3 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is predominantly expressed in the adult kidney, testis and fetal kidney and weaker expression in placenta, ovary, prostate, small intestine, and skeletally-derived cells. WISP-3 is required for normal postnatal skeletal growth and cartilage homeostasis. Human WISP-3 is a 36.8 kDa protein containing 331 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cystine knot-like (CTCK) domain |
| Source : |
E. Coli |
| Amino Acid Sequence : |
TGPLDTTPEG RPGEVSDAPQ RKQFCHWPCK CPQQKPRCPP GVSL VRDGCG CCKICAKQPG EICNEADLCD PHKGLYCDYS VDRPRYETGV CAYLVAVGCE FNQVHYHNGQ VFQPNPLFSC LCVSGAIGCT PLFIP KLAGS HCSGAKGGKK SDQSNCSLEP LLQQLSTSYK TMPAYRNLPL IWKKKCLVQA TKWTPCSRTC GMGISNRVTN ENSNCEMRKE KRLC YIQPCD SNILKTIKIP KGKTCQPTFQ LSKAEKFVFS GCSSTQSYKP TFCGICLDKR CCIPNKSKMI TIQFDCPNEG SFKWKMLWIT SCVC QRNCRE PGDIFSELKI L |
| Supplied : |
Lyophilized powder from with no additives. |
| Purity : |
>98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein. |
| Reconstitution : |
Lyophilized material should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4°C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4°Cfor up to 1 week or at -20°Cfor future use. |
| Activity : |
The ED50was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50for this effect is 0.2-0.3 g/ml. |
| Note : |
If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml. |
| Storage : |
The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20°C. Reconstituted WISP-3 should be stored in working aliquots at -20°C. Avoid frequent freeze thaw cycles. |
