||Recombinant human SPG21 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW=37.1kDa.
||Spastic paraplegia 21(SPG21), also known as Maspardin, binds to the hydrophobic C-terminal amino acids of CD4 and may play a role as a negative regulatory factor in CD4-depenent T-cell activation. This protein is widely expressed in various tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Mutations in SPG21 cause Mast syndrome, an autosomal-recessive complicated form of hereditary spastic paraplegia characterized by dementia, thin corpus callosum and white matter abnormalities.
|Amino Acid Sequence:
||MGSSHHHHHH SSGLVPRGSH MGEIKVSPDY NWFRGTVPLK KIIVDDDDSK IWSLYDAGPR SIRCPLIFLP PVSGTADVFF RQILALTGWG YRVIALQYPV YWDHLEFCDG FRKLLDHLQL DKVHLFGASL GGFLAQKFAE YTHKSPRVHS LILCNSFSDT SIFNQTWTAN SFWLMPAFML KKIVLGNFSS GPVDPMMADA IDFMVDRLES LGQSELASRL TLNCQNSYVE PHKIRDIPVT IMDVFDQSAL STEAKEEMYK LYPNARRAHL KTGGNFPYLC RSAEVNLYVQ IHLLQFHGTK YAAIDPSMVS AEELEVQKGS LGISQEEQ.
||Liquid. In 20mM Tris-HCl buffer (pH8.0).
||>95% by SDS–PAGE.
||1mg/ml (determined by Bradford assay).
||<1.0 EU per 1μg of protein (determined by LAL method).
||Can be stored at +4°C short term (1-2weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.