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Recombinant Human Sepiapterin Reductase, His-tagged

Cat.No. : SPR-190H
Product Overview : Recombinant human SPR protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW=30.2kDa.
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Cat. No. : SPR-190H
Description : Sepiapterinreductase (SPR) belongs to the short-chain dehydrogenase/reductase (SDR) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. SPR is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in SPR cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures.
Source : E.Coli.
Amino Acid Sequence : MGSSHHHHHH SSGLVPRGSH MEGGLGRAVC LLTGASRGFG RTLAPLLASL LSPGSVLVLS ARNDEALRQL EAELGAERSG LRVVRVPADL GAEAGLQQLL GALRELPRPK GLQRLLLINN AGSLGDVSKG FVDLSDSTQV NNYWALNLTS MLCLTSSVLK AFPDSPGLNR TVVNISSLCA LQPFKGWALY CAGKAARDML FQVLALEEPN VRVLNYAPGP LDTDMQQLAR ETSVDPDMRK GLQELKAKGK LVDCKVSAQK LLSLLEKDEF KSGAHVDFYD K.
From : Liquid. In 20mM Tris-HCl buffer (pH 8.0).
Purity : > 95% by SDS – PAGE.
Concentration : 1mg/ml (determined by Bradford assay).
Endotoxin Level : <1.0 EU per 1μg of protein (determined by LAL method).
Storage : Can be stored at +4°C short term (1-2weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : SPR sepiapterin reductase (7, 8-dihydrobiopterin: NADP+ oxidoreductase) [ Homo sapiens ]
Synonyms : SPR; sepiapterin reductase (7,8-dihydrobiopterin: NADP+ oxidoreductase); SDR38C1; short chain dehydrogenase/reductase family 38C, member1; Sepiapterin reductase; EC 1.1.1.153
Gene ID : 6697
mRNA Refseq : NM_003124
Protein Refseq : NP_003115
MIM : 182125
UniProt ID : P35270
Chromosome Location : 2p14-p12
Pathway : Folate biosynthesis; Metabolic pathways
Function : NADP or NADPH binding; aldo-keto reductase activity; binding; oxidoreductase activity; sepiapterin reductase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How to study the biological function of SPR proteins? 11/07/2019

Studying the biological function of SPR proteins requires a variety of experimental techniques and methods, such as cell biology, molecular biology, biochemistry and genomics. By observing gene expression, cell proliferation, signal transduction and other indicators, the biological function of SPR protein in cells can be evaluated.

What are the possible applications of SPR protein in future medical and scientific research? 08/27/2019

With the in-depth understanding of the structure and function of SPR protein, the drug development and application of SPR protein will be more extensive in the future. For example, the development of drugs that target the SPR protein may be used to treat tumors, autoimmune diseases, and neurological diseases. In addition, understanding the biological function of SPR proteins may also be useful for research in other areas of science.

What is the structure of the SPR protein? 07/13/2019

SPR proteins have multiple domains, including calcium-binding domains, kinase domains, and regulatory domains. Among them, the calcium ion binding domain can bind to calcium ions, the kinase domain can phosphorylate downstream proteins, and the regulatory domain can regulate the activity and stability of proteins.

How does SPR protein regulate gene expression regulation? 06/30/2019

SPR proteins can interact with a variety of transcription factors, such as NF-kB, AP-1, and CREB. Once interacting with these transcription factors, SPR proteins can regulate gene transcription and expression, influencing the biological behavior of cells.

What are the applications of SPR protein in drug discovery? 04/11/2019

Due to the important role of SPR protein in cell signal transduction and gene expression regulation, drug development targeting SPR protein is of great significance. At present, some drugs targeting SPR proteins have entered clinical trials for the treatment of some human diseases.

How are SPR proteins involved in cell signaling? 01/31/2019

During cell signaling, SPR proteins can interact with G protein-coupled receptors (GPCRs) and are activated by GPCRs. Once activated, SPR proteins can phosphorylate downstream proteins such as MAPK and AKT to further regulate intracellular signal transduction.

Customer Reviews (3)

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Reviews
12/09/2020

    The production process of SPR is very environmentally friendly, using clean production technology and green raw materials, so that the production process does not emit any harmful substances.

    10/24/2020

      This reproducibility of SPR is very good, and the results can be highly consistent in every experiment or application, which proves its excellent stability and reliability.

      10/06/2020

        This short half-life and high clearance of SPR provide new ideas and methods for the treatment of tumors, infection and other diseases.

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