Recombinant Human FK506 Binding Protein 1A, 12kDa, His-tagged FKBP1A-503H

Recombinant Human FK506 Binding Protein 1A, 12kDa, His-tagged

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Recombinant Human FK506 Binding Protein 1A, 12kDa, His-tagged

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Cat. No. : FKBP1A-503H
Product Overview : Recombinant human FKBP12 fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW=14.1kDa.
Description : FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1, 4, 5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor.
Source : E. Coli.
Amino Acid Sequence : MGSSHHHHHH SSGLVPRGSH MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE.
From : Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl, 1mM DTT, 10% glycerol.
Purity : >95% by SDS–PAGE.
Concentration : 1mg/ml (determined by Bradford assay).
Endotoxin Level : <1.0 EU per 1μg of protein (determined by LAL method ).
Biological activity : Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH 8.0 using chymotrypsin.
Activity Assay : 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant FKBP1a/FKBP12 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
Storage : Can be stored at +4°C short term (1-2weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1a7x. :

Gene Name : FKBP1A FK506 binding protein 1A, 12kDa [ Homo sapiens ]
Synonyms : FKBP1A; FK506 binding protein 1A, 12kDa; FKBP1; PKC12; PKCI2; FKBP12; PPIASE; FKBP-12; FKBP12C; FKBP1A; FK506-binding protein 1A (12kD); Peptidyl-prolyl cis-trans isomerase FKBP1A; PPIase FKBP1A; EC 5.2.1.8; FK506-binding protein 1A; FKBP-1A; Immunophilin FKBP12; 12 kDa FKBP; FKBP-12; OTTHUMP00000029978; Rotamase; rotamase; FK506-binding protein 1; FK506-binding protein 12; FK506-binding protein, T-cell, 12-kD; peptidyl-prolyl cis-trans isomerase; protein kinase C inhibitor 2
Gene ID : 2280
mRNA Refseq : NM_000801
Protein Refseq : NP_000792
MIM : 186945
UniProt ID : P62942
Chromosome Location : 20p13
Function : FK506 binding; FK506 binding; SMAD binding; activin binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity; ryanodine-sensitive calcium-release channel activity; signal transducer activity; transforming growth factor beta receptor activity; type I transforming growth factor beta receptor binding

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