Recombinant Human High-mobility Group Box 1, His-tagged HMGB1-532H

Recombinant Human High-mobility Group Box 1, His-tagged

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Recombinant Human High-mobility Group Box 1, His-tagged

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Cat. No. : HMGB1-532H
Product Overview : Recombinant human HMGB1 was expressed inHigh Five insect cellsand purified by conventional chromatography techniques. MW = 25kDa (223aa).
Description : High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor.
Source : High Fiveinsect cells.
Amino Acid Sequence : MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH.
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.5 mM DTT, 1 mM EDTA, 10% glycerol.
Purity : > 95% by SDS – PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDB rendering based on 1aab. :

Gene Name : HMGB1 high-mobility group box 1 [ Homo sapiens ]
Synonyms : HMGB1; DKFZp686A04236; HMG1; HMG3; SBP-1; HMG3; high-mobility group box 1; Amphoterin; OTTHUMP00000018199; OTTHUMP00000190860; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1; Amphoterin, High-Mobility Group Box 1
Gene ID : 3146
mRNA Refseq : NM_002128
Protein Refseq : NP_002119
MIM : 601912
UniProt ID : P09429
Chromosome Location : 13q12
Function : DNA bending activity; DNA binding; transcription factor binding

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