Recombinant Human Tumor Necrosis Factor

Cat. No.: TNF-194H
Product Overview: Recombinant human TNF-α was expressed inE.coliand purified by using conventional chromatography techniques. Additional amino acid, methionine, was attached at N-terminus of the protein. MW=17.5kDa (158 aa).
Description: Tumor necrosis factor alpha (TNF-α), also called cachectin, consists of 157 amino acids. TNF-α is a 17.5kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-α is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer.
Source: Escherichia Coli.
Amino Acids Sequences: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Purity: > 95% by SDS–PAGE.
Concentration: 1mg/ml (determined by Bradford assay).
Form: Liquid. In PBS buffer (pH7.4).
Endotoxin Level: < 1.0EU per 1 μg of protein (determined by LAL method).
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: TNF tumor necrosis factor (TNF superfamily, member 2) [ Homo sapiens ]
Synonyms: TNF; tumor necrosis factor (TNF superfamily, member 2); DIF; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; Tumor necrosis factor ligand; TNF-a; Cachectin; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; superfamily member 2; OTTHUMP00000029281; OTTHUMP00000037669; cachectin; APC1 protein; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha
Gene ID: 7124
mRNA Refseq: NM_000594
Protein Refseq: NP_000585
MIM: 191160
UniProt ID: P01375
Chromosome Location: 6p21.3
Pathway: receptor interaction; Fc epsilon RI signaling pathway; Graft-versus-host disease; Hypertrophic cardiomyopathy (HCM); Hematopoietic cell lineage; MAPK signaling pathway; Natural killer cell mediated cytotoxicity; RIG-I-like receptor signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway; TGF-beta signaling pathway; Toll-like receptor signaling pathway; Type I diabetes mellitus; Type II diabetes mellitus; Apoptosis; Hematopoietic cell lineage
Function: cytokine activity; identical protein binding; tumor necrosis factor receptor binding

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