||Recombinant human prokineticin-2 is an 8.8 kDa protein consisting of 81 amino acid residues and ten cysteine residues that potentially form five pairs of intra-molecular disulfide bonds. PROK2 was expressed inE. coli.
||Prokineticin-2 (PK2) is a cysteine-rich secreted protein that is expressed in the testis and in lower levels of the small intestine. PK2 regulates various biological functions including gastrointestinal motility, angiogenesis and circadiam rhythms. It is closely related to EG-VEGF (Prokineticin-1) and binds to two orphan B-protein-coupled receptors termed PK-R1 and PK-R2.
||AVITGACDKD SQCGGGMCCA VSIWVKSIRI CTPMGKLGDS CHPLTRKVPF FGRRMHHTC PCLPGLACLR TSFNRFICLA QK
||Lyophilized powder from with no additives.
||>98% by SDS-Page and HPLC analysis. Endotoxin level is less than 0.1ng per ug (1EU/ug) of protein.
||Lyophilized material should be reconstituted in water to a concentration of 0.1-1mg/ml. Allow to set at least 30 minutes at 4°C, mix well. If desired, tighten cap and centrifuge for 1 minute at 1000rpm to concentrate material in vial. This solution can be diluted into other buffered solutions or stored at 4°Cfor up to 1 week or at -20°Cfor future use. Note:If this material is not to be used for coating or labeling (or other applications requiring carrier free material), the addition of 0.1% BSA or HSA to dilution buffers or dilution in sterile culture media is recommended to enhance stability and minimize absorption to vial. Ideally, maintain stock >20ug/ml.
||The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20℃. Reconstituted Prokineticin-2 should be stored in working aliquots at -20°C.