Active Recombinant Human PROK2 protein, His-GST-tagged

• Cat.No.: PROK2-767H
• Product Overview: Active Recombinant Human PROK2 protein(Q9HC23)(30~128 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: 30-128 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Prokineticin 2 (PK2) is a secreted protein belonging to the prokineticins family. It thought to be involved in several important physiological processes like neurogenesis, tissue development, angiogenesis, and nociception. Otherimportant physiological roles the Bv8/Prokineticins (PKs) are involvedinmayinclude cancer, reproduction, and regulating physiological functions that influencecircadian rhythms like hormone secretion, ingestive behaviors, and the sleep/wakecycle. Besides, Prokineticin receptor 1(PKR1) has been identified as aninteractor of PK2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PK2 and recombinant human PKR1. Briefly, PK2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PKR1-coated microtiter wells and incubated for 2h at 37°C. Wellswere washed with PBST and incubated for 1h with anti-PK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PK2 and PKR1 was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 41.2kDa
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PROK2 prokineticin 2 [ Homo sapiens ]
• Official Symbol: PROK2
• Synonyms: PROK2; prokineticin 2; prokineticin-2; BV8; KAL4; MIT1; PK2; protein Bv8 homolog
• Gene ID: 60675
• mRNA Refseq: NM_001126128
• Protein Refseq: NP_001119600
• MIM: 607002
• UniProt ID: Q9HC23