Recombinant Human MMP19, Catalytic Domain

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Recombinant Human MMP19, Catalytic Domain

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Cat. No. : MMP19-161H
Product Overview : Recombinant Human matrix metalloproteinase-19 isorform 1 RASI-1 (MMP-19, Matrix metalloproteinase RASI, MMP-18) catalytic domain cloned from human cDNA was expressed inE.coli. The enzyme consists of the catalytic domain of human MMP-19, residues 101-256 with an additional methionine at N-term. Swiss prot accession: Q99542. MW=17.6kDa calculated.
Description : Matrix metalloproteinase-19 is an enzyme that in humans is encoded by the MMP19 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP"s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This protein is expressed in human epidermis and endothelial cells and it has a role in cellular proliferation, migration, angiogenesis and adhesion. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
Source : Escherichia Coli.
Purity : > 90% by SDS-PAGE. In an SDS-PAGE gel, the enzyme runs as a monomer <20kDa.
Specific Activity : >1.5U/μg. 1U=100pmol/min at 25ºC using a colorimetric assay with thiopeptolide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol).
Usage : Study enzyme kinetics, cleave target substrates and screen of inhibitors.
Supplied As : 0.2mg/ml in 20mM Tris, pH7.2, 10mM CaCl2, 0.1mM ZnCl2, 0.3M NaCl, 0.2M Acetohydroxamic Acid (AHA). The concentration is calculated by the analysis of the absorbance at 280nm (e280=25440M-1cm-1 calculated).
Storage : -80ºC. It is recommended that thawing and dilution of the enzyme be done in ice and within as short a time as possible before start of the assay. After initial defrost, aliquot product into individual tubes and refreeze at -80ºC. Avoid repeated freeze/defrost cycles.
Publication :
Gene Name : MMP19 matrix metallopeptidase 19 [ Homo sapiens ]
Synonyms : MMP19; matrix metallopeptidase 19; MMP18; RASI-1; MMP-19; MMP-18; matrix metalloproteinase 19; Matrix metalloproteinase-19; EC 3.4.24.-; Matrix metalloproteinase RASI; Matrix metalloproteinase-18; Matrix metalloproteinase 18
Gene ID : 4327
mRNA Refseq : NM_002429
Protein Refseq : NP_002420
MIM : 601807
UniProt ID : Q99542
Chromosome Location : 12q14
Function : calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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