||Recombinant Human matrix metalloproteinase-19 isorform 1 RASI-1 (MMP-19, Matrix metalloproteinase RASI, MMP-18) catalytic domain cloned from human cDNA was expressed inE.coli. The enzyme consists of the catalytic domain of human MMP-19, residues 101-256 with an additional methionine at N-term. Swiss prot accession: Q99542. MW=17.6kDa calculated.
||Matrix metalloproteinase-19 is an enzyme that in humans is encoded by the MMP19 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP"s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This protein is expressed in human epidermis and endothelial cells and it has a role in cellular proliferation, migration, angiogenesis and adhesion. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
||> 90% by SDS-PAGE. In an SDS-PAGE gel, the enzyme runs as a monomer <20kDa.
||>1.5U/μg. 1U=100pmol/min at 25ºC using a colorimetric assay with thiopeptolide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol).
||Study enzyme kinetics, cleave target substrates and screen of inhibitors.
||0.2mg/ml in 20mM Tris, pH7.2, 10mM CaCl2, 0.1mM ZnCl2, 0.3M NaCl, 0.2M Acetohydroxamic Acid (AHA). The concentration is calculated by the analysis of the absorbance at 280nm (e280=25440M-1cm-1 calculated).
||-80ºC. It is recommended that thawing and dilution of the enzyme be done in ice and within as short a time as possible before start of the assay. After initial defrost, aliquot product into individual tubes and refreeze at -80ºC. Avoid repeated freeze/defrost cycles.