Active Recombinant Human CD80 protein, Fc-His-tagged

• Cat.No.: CD80-589H
• Product Overview: Active Recombinant Human CD80 protein(Met 1-Asn 242)(NP_005182.1) was expressed in HEK293, fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc&His
• Protein Length: 1-242 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to bind human CD28 in a functional ELISA.
• Molecular Mass: The recombinant human B7-1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 455 amino acids and predicts a molecular mass of 51.7 kDa. By SDS-PAGE, the apparent molecular mass of rh B7-1/Fc is approximately 80-90 kDa due to the glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CD80 CD80 molecule [ Homo sapiens ]
• Official Symbol: CD80
• Synonyms: CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) , CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1
• Gene ID: 941
• mRNA Refseq: NM_005191
• Protein Refseq: NP_005182
• MIM: 112203
• UniProt ID: P33681

Citation

Antigen-Specific Suppression and Immunological Synapse Formation by Regulatory T Cells Require the Mst1 Kinase

Journal: PLoS ONE    PubMed ID: 24040101    Data: 2022/12/14

Authors: Takashi Tomiyama, Yoshihiro Ueda, Jacques Zimmer

Article Snippet:ICAM-1 liposomes were mixed with DOPC/CAP-biotin-PE liposomes containing 1 mol% DGS-NTA-Ni at a 2:1 ratio and deposited onto piranha-treated cover glass mounted on a 35 mm dish.ICAM-1 liposomes were mixed with DOPC/CAP-biotin-PE liposomes containing 1 mol% DGS-NTA-Ni at a 2:1 ratio and deposited onto piranha-treated cover glass mounted on a 35 mm dish.. Lipid bilayers on the glass were blocked with 1% BSA and loaded sequentially with Alexa488-labeled streptavidin (1 μg/ml) and mono-biotinylated I-A b with 0.1 M OVA peptide (AAHAEINEA) obtained through the NIH Tetramer Core Facility (Atlanta, GA) and CD80-histidine tag (Creative BioMart, Shirley, NY).. Densities of ICAM-1 (250 molecules/μm 2 ) and CD80 (90 molecules/μm 2 ) were measured as described [ ], and supported pMHC-dependent attachment and IS formation on lipid bilayers.Densities of ICAM-1 (250 molecules/μm 2 ) and CD80 (90 molecules/μm 2 ) were measured as described [ ], and supported pMHC-dependent attachment and IS formation on lipid bilayers.