Active Recombinant Human CD80 protein, His-tagged

• Cat.No.: CD80-591H
• Product Overview: Active Recombinant Human CD80 protein(P33681)(Val35-Asn242) is expressed in HEK293 with a 6His tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 35-242 aa
• Form: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Loaded Biotinylated Human CTLA-4-Fc-Avi on Protein A Biosensor, can bind Human B7-1-His with an affinity constant of 0.18 uM as determined in BLI assay.
• Molecular Mass: 24.7 KDa
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Publications:
  • Antigen-Specific Suppression and Immunological Synapse Formation by Regulatory T Cells Require the Mst1 Kinase (2013)

Gene Information

• Gene Name: CD80 CD80 molecule [ Homo sapiens ]
• Official Symbol: CD80
• Synonyms: CD80; CD80 molecule; CD28LG, CD28LG1, CD80 antigen (CD28 antigen ligand 1, B7 1 antigen) , CD80 molecule; T-lymphocyte activation antigen CD80; B lymphocyte activation antigen B7; B7 1; B7.1; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); B7; BB1; B7-1; LAB7; CD28LG; CD28LG1
• Gene ID: 941
• mRNA Refseq: NM_005191
• Protein Refseq: NP_005182
• MIM: 112203
• UniProt ID: P33681