Recombinant Human CD48 protein, His-tagged CD48-654H

Recombinant Human CD48 protein, His-tagged

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Recombinant Human CD48 protein, His-tagged

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Cat.No. : CD48-654H
Product Overview : Recombinant Human CD48 (Gln27-Ser220) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : CD48 antigen is also known as Signaling lymphocytic activation molecule 2, SLAM family member 2 (SLAMF2), Leukocyte antigen MEM-102, BCM1 surface antigen, B-lymphocyte activation marker BLAST-1. CD48 is a 65 kDa GPI-linked protein which belongs to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 antigen contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domain. CD48 interacts with CD244 in an heterophilic manner. CD48 is ligand for CD2. CD48 might facilitate interaction between activated lymphocytes. CD48 is probably involved in regulating T-cell activation.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Gln27
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 23.2 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Gln27-Ser220
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD48-654H :

Human CD48, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of CD48-654H :

Immobilized Human 2B4, Fc Tag at 5 μg/mL (100 μL/well) can bind Human CD48, His Tag with a linear range of 0.156-2.5 μg/mL (QC tested).

Gene Name : CD48
Official Symbol : CD48
Synonyms : CD48; CD48 molecule; BCM1, CD48 antigen (B cell membrane protein) , CD48 molecule; CD48 antigen; BLAST; hCD48; mCD48; SLAMF2; TCT.1; BCM1 surface antigen; leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); BCM1; BLAST1; MEM-102
Gene ID : 962
mRNA Refseq : NM_001256030
Protein Refseq : NP_001242959
MIM : 109530
UniProt ID : P09326

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