Recombinant Human NTRK2 protein, His-tagged NTRK2-157H

Recombinant Human NTRK2 protein, His-tagged

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Cat.No. : NTRK2-157H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human NTRK2 protein (Cys32-His430) was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Neurotrophic tyrosine kinase receptor type 2 (NTRK2) is also known as BDNF/NT-3 growth factors receptor, Tropomyosin-related kinase B (TRKB) and TrkB tyrosine kinase, which belongs to the protein kinase superfamily or Tyr protein kinase family. Insulin receptor subfamily. NTRK2/TrkB contains two Ig-like C2-type (immunoglobulin-like) domains, two LRR (leucine-rich) repeats, one LRRCT domain, one LRRNT domain, one protein kinase domain. NTRK2/Trk-B is expressed in the central and peripheral nervous system. The catalytic activity of NTRK2 is "ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate". NTRK2/TrkB involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Cys 32
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 45.2 kDa. The protein migrates as 60-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Cys32-His430
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of NTRK2-157H :

Human TrkB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : NTRK2
Official Symbol : NTRK2
Synonyms : OBHD; TRKB; trk-B; EIEE58; GP145-TrkB
Gene ID : 4915
mRNA Refseq : NM_001007097.3
Protein Refseq : NP_001007098.1
MIM : 600456
UniProt ID : Q16620
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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