Active Recombinant Human SOST protein, His-tagged

• Cat.No.: SOST-870H
• Product Overview: Recombinant Human SOST (NP_079513.1) (Gln24-Tyr213) was expressed in HEK293, fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln24-Tyr213
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit Wnt­3a­ induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is typically 1.5-6μg/mL in the presence of 3ng/mL Recombinant Mouse Wnt­3a. Measured by its ability to inhibit BMP­6 ­induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. 30µg/mL of rhSOST will antagonize hBMP­6 induction of alkaline phosphatase in MC3T3E1 cells by 50­80%.
• Molecular Mass: The recombinant human SOST is a monomeric protein consists of 197 amino acids and predicts a molecular mass of 22.5 kDa. As a result of glycosylation, the apparent molecular mass of rhSOST is approximately 29.9 kDa in SDS-PAGE under reducing conditions..
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  Sulfated hyaluronan improves bone regeneration of diabetic rats by binding sclerostin and enhancing osteoblast function (2016)
  Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone (2015)

Gene Information

• Gene Name: SOST sclerostin [ Homo sapiens ]
• Official Symbol: SOST
• Synonyms: SOST; sclerostin; sclerosteosis; VBCH
• Gene ID: 50964
• mRNA Refseq: NM_025237
• Protein Refseq: NP_079513
• MIM: 605740
• UniProt ID: Q9BQB4

Citation

Sulfated hyaluronan improves bone regeneration of diabetic rats by binding sclerostin and enhancing osteoblast function.

Journal: Biomaterials    PubMed ID: 27131598    Data: 2017/12/18

Authors: Ann-Kristin Picke, Juliane Salbach-Hirsch, Lorenz C Hofbauer

Article Snippet:Fluoraldehyde? ophthaldialdehyde reagent solution was purchased from Thermo Fisher Scientific (Schwerte, Germany).Fluoraldehyde? ophthaldialdehyde reagent solution was purchased from Thermo Fisher Scientific (Schwerte, Germany).. Recombinant human sclerostin was obtained from Creative BioMart (SOST870H, New York, USA) and D-(+) glucose from Sigma-Aldrich (St. Louis, USA).. The Cell Death Detection ELISA and Cell Proliferation ELISA, BrdU were acquired from Roche (Basel, Switzerland) and the Caspase-Glo? 3/7 Assay from Promega (Madison, USA).The Cell Death Detection ELISA and Cell Proliferation ELISA, BrdU were acquired from Roche (Basel, Switzerland) and the Caspase-Glo? 3/7 Assay from Promega (Madison, USA).

Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone.

Journal: Biomaterials    PubMed ID: 26232882    Data: 2016/5/23

Authors: Juliane Salbach-Hirsch, Sergey A Samsonov, Lorenz C Hofbauer

Article Snippet:HA (from Streptococcus, MW? 1.1 106 gmol 1) was purchased from Aqua Biochem (Dessau, Germany), pentylamine-biotin from Thermo Fisher Scientific (Bonn, Germany).HA (from Streptococcus, MW? 1.1 106 gmol 1) was purchased from Aqua Biochem (Dessau, Germany), pentylamine-biotin from Thermo Fisher Scientific (Bonn, Germany).. Recombinant human sclerostin (SOST-870H) was obtained from Creative BioMart (New York, USA). . The Series S Sensor Chips C1, the amine coupling kit and HBS-EP were purchased from GE Healthcare Europe GmbH (Freiburg, Germany).The Series S Sensor Chips C1, the amine coupling kit and HBS-EP were purchased from GE Healthcare Europe GmbH (Freiburg, Germany).