| Description : |
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH 8.5, 150 mM NaCl. |
| Molecular Mass : |
Approximately 31.5 kDa, a single non-glycosylated polypeptide chain containing 288 amino acids. |
| Protein length : |
288 |
| AA Sequence : |
GNCWLRQAKNGRCQVLYKTELSKEE CCSTGRLSTSWTEEDVNDNTLFKWM IFNGGAPNCIPCKETCENVDCGPGK KCRMNKKNKPRCVCAPDCSNITWKG PVCGLDGKTYRNECALLKARCKEQP ELEVQYQGRCKKTCRDVFCPGSSTC VVDQTNNAYCVTCNRICPEPASSEQ YLCGNDGVTYSSACHLRKATCLLGR SIGLAYEGKCIKAKSCEDIQCTGGK KCLWDFKVGRGRCSLCDELCPDSKS DEPVCASDNATYASECAMKEAACSS GVLLEVKHSGSCN |
| Endotoxin : |
Less than 1 EU/µg of rHuFollistatin as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
