Recombinant Human Cardiotrophin-like Cytokine Factor 1

• Cat.No.: CLCF1-953H
• Product Overview: Recombinant human NNT-1/BCSF-3 produced inE.Coliis a 22.4 kDa protein containing 199 amino acid residues.

Specification

• Cat. No.: CLCF1-953H
• Description: NNT-1/BCSF-3 is a neurotrophic factor with B-cell stimulating capabilities. Expressed in lymph nodes and spleen, NNT-1/BCSF-3 binds and activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member beta (LIFR-beta) and induces tyrosine phosphorylation of these receptors. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss and B cell hyperplasia associated with increased in serum IgG and IgM.
• Source: E.Coli.
• Purity: > 98% by SDS-PAGE and HPLC analysis.
• Endotoxin Level: < 0.1 ng per μg of NNT-1/BCSF-3.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biological Activity: NT-1/BCSF-3 weakly supports chick E8 DRG neurite outgrowth at a concentration of 1.0 ng/ml.
• Reconstitution: The human NNT-1/BCSF-3 should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.
• Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted human NNT-1/BCSF-3 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Pathways: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway

Gene Information

• Gene Name: CLCF1 cardiotrophin-like cytokine factor 1 [ Homo sapiens ]
• Synonyms: cardiotrophin-like cytokine factor 1; CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1; CLCF1; novel neurotrophin-1; B-cell stimulating factor 3; B-cell stimulatory factor 3; CRLF1 associated cytokine-like factor 1; neurotrophin-1/B-cell stimulating factor-3; B cell-stimulating factor 3; cold-induced sweating syndrome 2
• Gene ID: 23529
• mRNA Refseq: NM_001166212
• Protein Refseq: NP_001159684
• MIM: 607672
• UniProt ID: Q9UBD9
• Chromosome Location: 11q13.3
• Function: cytokine activity; growth factor activity