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||N-terminal GST-tagged full-length protein. MKK6 is one of the major specific activators of p38. MAP kinase kinases (MAPKKs) MKK3 and MKK6 are the primary regulators of p38 phosphorylation and activation. The MKK6/p38 pathway is well documented in the regulation of apoptosis. The p38 mitogen-activated protein (MAP) kinase signal transduction pathway regulates the production of interleukin-1 and tumor necrosis factor-alpha. The recombinant human MKK6 was expressed inE. coliand purified by glutathione sepharose chromatography. It is suitable for labeling MKK6 substrates. The highly active form is produced by two amino acid exchanges (S151E, T155E). The sequence based calculated molecular weight is 63.64 kDa.
||Supplied in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 1 mM DTT and 270 mM sucrose.
||> 95% by SDS-PAGE.
||> 50,000 U/mg (1 Unit is defined as the amount of MKK6 which activates inactive p38α/SAPK2a (0.5 mg/ml) by 1 U/min using 125 μM ATP at 30 °C. 1 Up38α/SAPK2a activity is defined as 1 picomole phosphate transferred to myelin basic protein per min at 30 °C).
||For in vitro use only.
||Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.