||LRH-1 (NR5A2) is expressed specifically in pancreas and liver, playing important roles in the regulation of several liver-specific genes. LRH-1 is a mammalian homologue of Drosophila fushi tarazu factor (dFTZ-F1) and structurally belongs to the orphan nuclear receptor superfamily. The liver-specific activity of ENII is regulated by multiple liverenriched transcription factors, including LRH-1/hB1F. The role LRH-1 and dosagesensitive sex reversal, adrenal hypoplasia congenital critical region on the X chromosome, gene 1 (DAX-1) in the regulation of StAR gene expression in human granulosa cell tumor cells has been investigated. LRH-1, is expressed in granulosa cells and has been shown to synergize with the cAMP signaling system to regulate the gonadal type II aromatase promoter in transient transfection assays. Observations support a direct role for LRH-1 in the induction of the progesterone but not the estrogen biosynthetic pathway during granulose cell differentiation. The human nuclear receptor liver receptor homolog 1 (hLRH-1) plays an important role in the development of breast carcinomas. Results indicate that hLRH-1's control of gene expression is mediated by phospholipid binding, and establish hLRH-1 as a novel target for compounds designed to slow breast cancer development.
||Purified LRH-1 can be used for DNA, protein-protein interaction, and gel mobility shift assays.
||For in vitro use only.
||Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
||> 95% by SDS-PAGE.
||Quality guaranteed for 12 months, Store at -80°C. Avoid freeze / thaw cycles.
||Maturity onset diabetes of the young; Gene Expression; Regulation of beta-cell development