||Recombinant Human PNPLA3encoding the human PNPLA3 (Met1–Leu481) was expressed inE.coli.It was recombined with a Hexa-his tag at the N-Terminus and purified by Ni-NTA column. MW=53.0 KD.
||Patatin-like phospholipase domain-containing protein 3 is an enzyme that in humans is encoded by the PNPLA3 gene. The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes.
||Human PNPLA3 (Met1–Leu481)1 MYDAERGWSL SFAGCGFLGF YHVGATRCLS EHAPHLLRDA RMLFGASAGA LHCVGVLSGI PLEQTLQVLS DLVRKARSRN IGIFHPSFNL SKFLRQGLGK CLPANVHQLI SGKIGISLTR VSDGENVLVS DFRSKDEVVD ALVCSCFMPF YSGLIPPSFR GVRYVDGGVS DNVPFIDAKT TITVSPFYGE YDICPKVKST NFLHVDITKL SLRLCTGNLY LLSRAFVPPD LKVLGEICLR GYLDAFRFLE EKGICNRPQP GLKSSSEGMD PEVAMPSWAN MSLDSSPESA ALAVRLEGDE LLDHLRLSIL PWDESILDTL SPRLATALSE EMKDKGGYMS KICNLLPIRI MSYVMLPCTL PVESAIAIVQ RLVTWLPDMP DDVLWLQWVT SQVFTRVLMC LLPASRSQMP VSSQQASPCT PEQDWPCWTP CSPEGCPAET KAEATPRSIL RSSLNFFLGN KVPAGAEGLS TFPSFSLEKS 481
||Lyophilized 100μg human PNPLA3 in 50μl of TBS (20mM Tris, 50mM NaCl, pH8.0). Carry free.
||>95% by SDS gel.
||Add 500μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
||Store lyophilized protein at -20℃ or -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at -20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.