Recombinant Human Granulin, His-tagged GRN-113H

Recombinant Human Granulin, His-tagged

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Recombinant Human Granulin, His-tagged

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Cat. No. : GRN-113H
Product Overview : Recombinant Human granulin encoding the human Pro-Granulins(Thr18-Leu593) was expressed inE.coli. It was recombined with a Hexa-his sequence of pET-28a vector at the N-Terminusand purified by Ni-NTA column. MW= 63.4 KD.
Description : Granulin is a protein that in humans is encoded by the GRN gene.Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Sequence : Human Pro-Granulins (Thr18-Leu593)18 TRCPDGQFCP VACCLDPGGA SYSCCRPLLD KWPTTLSRHL GGPCQVDAHC SAGHSCIFTV SGTSSCCPFP EAVACGDGHH CCPRGFHCSA DGRSCFQRSG NNSVGAIQCP DSQFECPDFS TCCVMVDGSW GCCPMPQASC CEDRVHCCPH GAFCDLVHTR CITPTGTHPL AKKLPAQRTN RAVALSSSVM CPDARSRCPD GSTCCELPSG KYGCCPMPNA TCCSDHLHCC PQDTVCDLIQ SKCLSKENAT TDLLTKLPAH TVGDVKCDME VSCPDGYTCC RLQSGAWGCC PFTQAVCCED HIHCCPAGFT CDTQKGTCEQ GPHQVPWMEK APAHLSLPDP QALKRDVPCD NVSSCPSSDT CCQLTSGEWG CCPIPEAVCC SDHQHCCPQG YTCVAEGQCQ RGSEIVAGLE KMPARRASLS HPRDIGCDQH TSCPVGQTCC PSLGGSWACC QLPHAVCCED RQHCCPAGYT CNVKARSCEK EVVSAQPATF LARSPHVGVK DVECGEGHFC HDNQTCCRDN RQGWACCPYR QGVCCADRRH CCPAGFRCAA RGTKCLRREA PRWDAPLRDP ALRQLL 593
Source : E.coli.
Formulation : Lyophilized 100μg human GRN in 50μl of TBS (20mM Tris, 50mM NaCl, pH8.0). Carry free.
Purity : >95% by SDS gel.
Application : Cell biology.
Reconstitution : Add 500μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
Storage : Store lyophilized protein at -20℃ or -70℃. Lyophilized protein is stable for up to 6 months from date of receipt at -20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
Gene Name : GRN granulin [ Homo sapiens ]
Synonyms : GRN; granulin; GEP; GP88; PEPI; PGRN; PCDGF; progranulin; Granulins; Proepithelin; Acrogranin; Paragranulin; Granulin-1; Granulin G; Granulin-2; Granulin F; Granulin-3; Granulin B; Granulin-4; Granulin A; Granulin-5; Granulin C; Granulin-6; Granulin D; Granulin-7; Granulin E; PC cell-derived growth factor; granulin-epithelin
Gene ID : 2896
mRNA Refseq : NM_002087
Protein Refseq : NP_002078
MIM : 138945
UniProt ID : P28799
Chromosome Location : 17q21.32
Function : cytokine activity; growth factor activity

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