||Recombinant Human Gastrokine 1 encoding the Mature Human Gastrokine-1 (Asn21-Asn185P) with 6 His tag on the N-Terminus was expressed inE.Coli.This protein was purified by Ni-NTA column. MW=20KD.
||Gastrokine-1 is a protein that in humans is encoded by the GKN1 gene. The protein encoded by this gene is found to be down-regulated in human gastric cancer tissue as compared to normal gastric mucosa.
||Mature human Gastrokine-1 (Asn21-Asn185P)NYNINVNDDN NNAGSGQQSV SVNNEHNVAN VDNNNGWDSW NSIWDYGNGF AATRLFQKKT CIVHKMNKEV MPSIQSLDAL VKEKKLQGKG PGGPPPKGLM YSVNPNKVDD LSKFGKNIAN MCRGIPTYMA EEMQEASLFF YSGTCYTTSV LWIVDISFCG DTVEN.
||>95% in SDS gel.
||Lyophilized 100μg Mature human Gastrokine-1 (Asn21-Asn185P) in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
||Cell biology, ELISA.
||Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
||Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.