||Recombinant Human Growth Differentiation Factor 2 encoding the human Growth factor 2 Procursor (Met1-Arg429) was recombined with his tag at the N-Terminus. That was expressed inE.Coli,and purified by Ni-NTA column. MW=47.3KD.
||Growth differentiation factor 2 (GDF2) is a protein also known as bone morphogenetic protein (BMP)-9 that belongs to the transforming growth factor beta superfamily. It has a role in inducing and maintaining the ability of embryonic basal forebrain cholinergic neurons (BFCN) to respond to a neurotransmitter called acetylcholine; BFCN are important for the processes of learning, memory and attention. GDF2 is also important for the maturation of BFCN. Another role of GDF2 has been recently suggested. GDF2 is a potent inducer of hepcidin (a cationic peptide that has a antimicrobial properties) in liver cells (hepatocytes) and can regulate iron metabolism.
||Human Growth differentiation factor 2(Met1-Arg429)1MCPGALWVAL PLLSLLAGSL QGKPLQSWGR GSAGGNAHSP LGVPGGGLPE HTFNLKMFLE NVKVDFLRSL NLSGVPSQDK TRVEPPQYMI DLYNRYTSDK STTPASNIVR SFSMEDAISI TATEDFPFQK HILLFNISIP RHEQITRAEL RLYVSCQNHV DPSHDLKGSV VIYDVLDGTD AWDSATETKT FLVSQDIQDE GWETLEVSSA VKRWVRSDST KSKNKLEVTV ESHRKGCDTL DISVPPGSRN LPFFVVFSND HSSGTKETRL ELREMISHEQ ESVLKKLSKD GSTEAGESSH EEDTDGHVAA GSTLARRKRS AGAGSHCQKT SLRVNFEDIG WDSWIIAPKE YEAYECKGGC FFPLADDVTP TKHAIVQTLV HLKFPTKVGK ACCVPTKLSP ISVLYKDDMG VPTLKYHYEG MSVAECGCR 429.
||>95% in SDS gel.
||Lyophilized 100 μg human Growth differentiation factor 2 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
||Cell biology, ELISA.
||Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
||Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.