|Cat. No. :
|Product Overview :
||Recombinant Human neuregulin 4 encoding the human sNRG4 was recombined with Hexa-histidine tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=6.7 KD.
||sNRG4 is a low affinity ligand for the ERBB4 tyrosine kinase receptor. It concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors sNRG4 does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
||>95% in SDSgel.
||Lyophilized 100 μg human NRG4 in 50 μl of TBS ( 20 mM Tris, 50mM NaCl, pH8.0 ). Carry free.
||Add 500μl deionized water or TBS to the vial to prepare a working stock solution at 200 μg/mL. Allow to set at least 30 minutes at 4℃, mix well.
||Cell biology, ELISA.
||Store lyophilized protein at -20℃ to-70℃. Lyophilized protein is stable for up to 6 months from date of receipt at - 20℃ to -70℃. Upon reconstitution, this protein can be stored at -20℃ for up to a few weeks or at -70℃ in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freeze / thaw cycles.