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||CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. PCAF belongs to the GCN5/PCAF family of nuclear HATs. The proteins consist of N-terminal and C-terminal domains separated by a deep hydrophobic cleft. The C-terminus contains the HAT domain and a bromodomain, while the Nterminus contains a PCAF-specific domain. Numerous studies indicate that these HATs function as histone-acetylating transcriptional co-activators. Besides histones, PCAF also acetylates non-histone proteins. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.
||Sf9 insect cells.
||Liquid. Supplied in 20mM Tris-HCl pH8.0, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT.
||1-5ng are sufficient for a gel mobility shift assay in a 20μl reaction, 50-100ng are sufficient for reconstituted transcription assay and 100-200ng are sufficient for a protein-protein interaction assay or an acetylation assay.
||Use only for Research. Recombinant PCAF can be used: (1) for protein-protein raction assay. (2)For in vitro transcription assay. (3) For in vitro acetylation assay. (4) For cell growth assay.
||>95% by SDS-PAGE.
||For in vitro use only.
||Quality guaranteed for 12 months. Store at -80℃. Avoid freeze / thaw cycles.