Active Recombinant Human SIRT3

• Cat.No.: SIRT3-341H

Specification

• Cat. No.: SIRT3-341H
• Description: Sirtuins are pylogenetically conserved from bacteria to humans and regulate cell functions beyond silencing. The proteins show a relevance to cancer, obesity, muscle differentiation, inflammation and neuro-degeneration. In addition, Sirtuin activity may extend the lifespan of several organisms. Human Sirtuin 3 (Sirt3) is a mitochondrial member of Class III of the protein deacetylases family (historically called histone deacetylases family). Class III deacetylases utilize NAD+ as cosubstrate and act either as deacetylases or as ADP-ribosyltransferases. Sirt3 regulates mitochondrial function and thermogenesis; the enzyme can deacetylate and thereby activate the mitochondrial acetyl-CoA synthetase 2 which enables mitochondria to activate acetate for metabolic reactions. MW=32.2kDa (292 amino acids).
• Source: E.coli.
• Form: Purified recombinant protein in 20mM Tris-HCl pH7.8, and 150mM NaCl.
• Activity: >20units/μg 1 unit is defined as that amount of enzyme required to catalyze 1 pmol of product per minute at 37℃, 100μM Fluor de Lys-SIRT2 Substrate (Biomol) and 500μM NAD+.
• Purity: >90% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.
• Publication:
  Mechanism of Inhibition of the Human Sirtuin Enzyme SIRT3 by Nicotinamide: Computational and Experimental Studies (2014)

Gene Information

• Gene Name: SIRT3 sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) [ Homo sapiens ]
• Synonyms: SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); SIR2L3; NAD-dependent deacetylase sirtuin-3, mitochondrial; EC 3.5.1.-; SIR2-like protein 3; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase 2; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; sirtuin 3; sirtuin type 3
• Gene ID: 23410
• mRNA Refseq: NM_001017524
• Protein Refseq: NP_001017524
• MIM: 604481
• UniProt ID: Q9NTG7
• Chromosome Location: 11p15.5
• Function: NAD binding; NAD+ ADP-ribosyltransferase activity; hydrolase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; metal ion binding; protein binding; zinc ion binding