Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Mouse Vascular Endothelial Growth Factor A

Cat.No. : Vegfa-313M
Product Overview : Recombinant Mouse VEGF-A protein was expressed in E.coli and purified by using conventional chromatography techniques. MW=16.3 kDa (141aa).
  • Specification
  • Gene Information
  • Related Products
Cat. No. : Vegfa-313M
Description : VEGF-A, also known as vascular endothelial growth factor, belong to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis.
Source : E. Coli.
Form : Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
Molecular Weight : 16.3 kDa (141aa), confirmed by MALDI-TOF
Purity : > 90% by SDS-PAGE.
Concentration : 0.5 mg/ml (determined by Bradford assay).
Biological Activity : Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml.
Activity Assay : 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast ) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul) 5. Incubation time: 24 hr (after sample treatment) 6. Concentration range: 1 pg/ml – 1 ug/ml 7. Detection method : BrdU assay
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : Vegfa vascular endothelial growth factor A [ Mus musculus ]
Synonyms : Vegfa; vascular endothelial growth factor A; Vpf; Vegf; Vegf-a; Vegf120; Vegf164; Vegf188; VPF; VEGF-A; MGC70609; MVCD1; VEGF; Vascular permeability factor; vascular endothelial growth factor; vascular endothelial growth factor isoform VEGF165
Gene ID : 22339
mRNA Refseq : NM_001025250
Protein Refseq : NP_001020421
UniProt ID : Q00731
Chromosome Location : 17 24.2cM
Function : growth factor activity; heparin binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
How does VEGFA protein relate to other biomarkers? 05/28/2022

Studies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.

How to harness the activity of VEGFA protein for disease treatment? 05/14/2022

At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.

What is the role of VEGFA protein in tumor angiogenesis? 09/24/2020

VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.

How well is the VEGFA protein expressed in different tissues and organs? 07/09/2020

The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.

What is the relationship between the expression level of VEGFA protein and the degree of tumor malignancy? 03/26/2020

Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.

How about the fuction of VEGFA protein in cardiovascular disease? 01/10/2019

VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.

Customer Reviews (2)

Write a review
Reviews
04/21/2021

    VEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.

    03/09/2020

      VEGFA they produced was highly active and the experimental effect was obvious.

      Ask a Question for All Vegfa Products

      Required fields are marked with *

      My Review for All Vegfa Products

      Required fields are marked with *

      0

      Inquiry Basket

      cartIcon
      logo

      FOLLOW US

      Terms and Conditions        Privacy Policy

      Copyright © 2024 Creative BioMart. All Rights Reserved.

      Contact Us

      • /

      Stay Updated on the Latest Bioscience Trends