Recombinant Mouse Vascular Endothelial Growth Factor A
Cat.No. : | Vegfa-313M |
Product Overview : | Recombinant Mouse VEGF-A protein was expressed in E.coli and purified by using conventional chromatography techniques. MW=16.3 kDa (141aa). |
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- Gene Information
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Cat. No. : | Vegfa-313M |
Description : | VEGF-A, also known as vascular endothelial growth factor, belong to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis. |
Source : | E. Coli. |
Form : | Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol. |
Molecular Weight : | 16.3 kDa (141aa), confirmed by MALDI-TOF |
Purity : | > 90% by SDS-PAGE. |
Concentration : | 0.5 mg/ml (determined by Bradford assay). |
Biological Activity : | Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml. |
Activity Assay : | 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast ) 2. Maintenance Condition: RPMI 1640 containing 10% FBS 3. Assay medium: serum free RPMI 1640 4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul) 5. Incubation time: 24 hr (after sample treatment) 6. Concentration range: 1 pg/ml – 1 ug/ml 7. Detection method : BrdU assay |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | Vegfa vascular endothelial growth factor A [ Mus musculus ] |
Synonyms : | Vegfa; vascular endothelial growth factor A; Vpf; Vegf; Vegf-a; Vegf120; Vegf164; Vegf188; VPF; VEGF-A; MGC70609; MVCD1; VEGF; Vascular permeability factor; vascular endothelial growth factor; vascular endothelial growth factor isoform VEGF165 |
Gene ID : | 22339 |
mRNA Refseq : | NM_001025250 |
Protein Refseq : | NP_001020421 |
UniProt ID : | Q00731 |
Chromosome Location : | 17 24.2cM |
Function : | growth factor activity; heparin binding |
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◆ Native Protein | ||
VEGFA-31701TH | Native Human VEGFA | +Inquiry |
◆ Lysates | ||
VEGFA-1427HCL | Recombinant Human VEGFA cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionStudies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.
At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.
VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.
The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.
Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.
VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.
Customer Reviews (2)
Write a reviewVEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.
VEGFA they produced was highly active and the experimental effect was obvious.
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