Recombinant Canine Clusterin, Flag-tagged CLU-1319C

Recombinant Canine Clusterin, Flag-tagged

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Recombinant Canine Clusterin, Flag-tagged

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Cat. No. : CLU-1319C
Product Overview : Recombinant Canine Apoliprotein-J produced inHEK293is a single, non-glycosylated, Polypeptide chain containing436 amino acids and having a molecular mass of 50.72 kDa. The protein is fused with 13 amino acid Flag tag at N-Terminus. The Apoliprotein-J Canine is purified by proprietary chromatographic techniques.
Description : Clusterin mRNA and Clusterin protein are shown to increase with androgen treatment. Binding of clusterin to the LDL-Receptor plays a role in the pathogenesis of membranous glomerulonephritis. Clusterin is down regulated in CaP in association with matched benign controls. Clusterin is involved in cellular senescence and tumorigenesis. Clusterin is involved in photo-oxidative cell death pathway. Clusterin is a functional tumor marker for the diagnosis of pediatric large cell lymphoma. Clusterin is activated in low pH. Clusterin is involved in the inhibition of NF-kappaB signaling through stabilization of IkappaBs thus results in suppression of tumor cell motility. N-terminal deletion of clusterin is vital for its alterations of biogenesis in esophageal squamous cell carcinoma.
Source : Human Embryonic Kidney 293 Cells.
Amino Acid Sequence : PGDYKDDDDK PAGDQAVSDT ELQEMSTEGS KYINKEIKNA LKGVKQIKTL IEQTNEERKS LLSNLEEAKK KKEDALNDTK DSETKLKASQ GVCNDTMMAL WEECKPCLKQ TCMKFYARVC RSGSGLVGHQ LEEFLNQSSP FYFWMNGDRI DSLLENDRQQ THALDVMQDS FNRASSIMDE LFQDRFFTRE PQDTYHYSPF SLFQRRPFFN PKFRIARNII PFPRFQPLNF HDMFQPFFDM IHQAQQAMDV NLHRIPYHFP IEFPEEDNRT VCKEIRHNST GCLKMKDQCE KCQEILSVDC SSNNPAQVQL RQELSNSLQI AEKFTKLYDE LLQSYQEKMF NTSSLLKQLN EQFSWVSQLA NLTQSEDPFY LQVTTVGSQT SDSNVPVGFT KVVVKLFDSD PITVMIPEAV SRNNPKFMET VAEKALQEYRQKHREE
Purity : Greater than 95% as determined by SDS PAGE.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation : Canine Clusterin was lyophilized from 0.5mg/ml solution containing 20mM Tris buffer and 20mM NaCl,pH7.5
Solubility : Add deionized H2O to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely.
Applications : ELISA, Western Blot.
Stability : Store lyophilized protein at -20℃. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4℃ for a limited period of time; it does not show any change after two weeks at 4℃. The lyophilized protein remains stable until the expiry date when stored at -20℃.
Gene Name : CLU clusterin [ Canis lupus familiaris ]
Synonyms : CLU; clusterin; CLU; GP80; glycoprotein 80; lusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
Gene ID : 442971
mRNA Refseq : NM_001003370
Protein Refseq : NP_001003370
UniProt ID : P25473
Chromosome Location : 25

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